Immobilization of albumin on aminosilane modified superparamagnetic magnetite nanoparticles and its characterization

被引：257

作者：

Can, Keziban ^{[1
]}

Ozmen, Mustafa ^{[1
]}

Ersoz, Mustafa ^{[1
]}

机构：

[1] Selcuk Univ, Dept Chem, TR-42075 Konya, Turkey

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2009年 / 71卷 / 01期

关键词：

Magnetic nanoparticles; APTES; Silanization; Immobilization; Albumin; DEXTRAN; IMMUNOMICROSPHERES; STABILIZATION; ANTIBODIES; SEPARATION; LAYER;

D O I：

10.1016/j.colsurfb.2009.01.021

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Magnetite nanoparticles (NPs) were synthesized by co-precipitating Fe2+ and Fe3+ in an ammonia solution. Magnetite NPs coated with 3-aminopropyltriethoxysilane (APTES) were prepared by silanization reaction. After activated with glutaraldehyde (CA), human serum albumin was covalently immobilized on these magnetic NPs. The influence of three different buffer solutions on covalent immobilization was studied. phosphate buffer saline solution (PBS) was shown the highest binding ability for immobilization process compared to other buffer solutions. The morphology and properties of these magnetic NPs were examined by transmission electron eicroscopy (TEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA) and magnetization measurement. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：154 / 159

页数：6

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