Simultaneous determination of creatinine, creatine, and guanidinoacetic acid in human serum and urine using liquid chromatography atmospheric pressure chemical ionization mass spectrometry

被引：43

作者：

Yasuda, M ^{[1
]}

Sugahara, K ^{[1
]}

Zhang, JY ^{[1
]}

Ageta, T ^{[1
]}

Nakayama, K ^{[1
]}

Shuin, T ^{[1
]}

Kodama, H ^{[1
]}

机构：

[1] KOCHI MED SCH, DEPT CHEM, KOCHI 783, JAPAN

来源：

ANALYTICAL BIOCHEMISTRY | 1997年 / 253卷 / 02期

关键词：

D O I：

10.1006/abio.1997.2343

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and direct method for simultaneous determination of creatinine, creatine, and guanidinoacetic acid in biological samples has been developed by using column liquid chromatography-mass spectrometry (LC/APCI-MS). The determinations of creatinine, creatine, and guanidinoacetic acid in the samples of human urine and serum were carried out by scanning the [M + H](+) ions of each compound. The recoveries of authentic compounds were 93.87 +/- 6.00% (n = 5) for creatinine, 94.80 +/- 8.93% (n = 5) for creatine, and 90.92 +/- 7.96% (n = 5) for guanidinoacetic acid after ion-exchange resin treatment. The content of creatinine in human urine and serum was also measured by the Jaffe method, a common method of determination of creatinine currently used in hospitals. The contents of creatinine, creatine, and guanidinoacetic acid obtained using LC/APCI-MS coincided well with those reported in previous papers. (C) 1997 Academic Press.

引用

页码：231 / 235

页数：5

共 14 条

[1] PREDICTORS OF RENAL-FUNCTION IN DIABETIC AND NON-DIABETIC RENAL-DISEASE [J].