Modulation of Strawberry/Cranberry Phenolic Compounds Glucuronidation by Co-Supplementation with Onion: Characterization of Phenolic Metabolites in Rat Plasma Using an Optimized μSPE-UHPLC-MS/MS Method

被引：27

作者：

Dudonne, Stephanie ^{[1
,2
]}

Dube, Pascal ^{[1
,2
]}

Pilon, Genevieve ^{[1
,3
]}

Marette, Andre ^{[1
,3
]}

Jacques, Helene ^{[1
,4
]}

Weisnagel, John ^{[5
]}

Desjardins, Yves ^{[1
,2
]}

机构：

[1] Univ Laval, Inst Nutr & Funct Foods INAF, Quebec City, PQ G1V 0A6, Canada

[2] Univ Laval, CRH, Quebec City, PQ G1V 0A6, Canada

[3] Univ Laval, Quebec Heart & Lung Inst CRIUCPQ, Dept Med, Quebec City, PQ G1V 4G5, Canada

[4] Univ Laval, Dept Food & Nutr Sci, Quebec City, PQ G1V 0A6, Canada

[5] Laval Univ Hosp Ctr CHUL, Dept Med, Quebec City, PQ G1V 4G2, Canada

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2014年 / 62卷 / 14期

关键词：

bioavailability; GlucoPhenol; mu SPE; phenolic metabolites; UHPLC-MS/MS; ANTHOCYANINS; POLYPHENOLS; QUERCETIN; BIOAVAILABILITY; ABSORPTION; INGESTION; HUMANS; FOODS; DEGLYCOSYLATION; IDENTIFICATION;

D O I：

10.1021/jf404965z

中图分类号：

S [农业科学];

学科分类号：

082806 [农业信息与电气工程];

摘要：

Plant phenolic compounds are suggested to exert pharmacological activities in regards to obesity and type-2 diabetes, but their mode of action is poorly understood due to a lack of information about their bioavailability. This work aimed to study the bioavailability of GlucoPhenol phenolic compounds, a strawberry cranberry extracts blend, by characterizing plasma phenolic profile in obese rats. A comparison was performed by co-supplementation with an onion extract. Using an optimized mu SPE-UHPLC-MS/MS method, 21 phenolic metabolites were characterized, mostly conjugated metabolites and microbial degradation products of the native phenolic compounds. Their kinetic profiles revealed either an intestinal or hepatic formation. Among identified metabolites, isorhamnetin glucuronide sulfate was found in greater amount in plasma. Three glucuronidated conjugates of strawberry cranberry phenolic compounds, p-hydroxybenzoic acid glucuronide, catechins glucuronide, and methyl catechins glucuronide were found in higher quantities when GlucoPhenol was ingested together with onion extract (+252%, +279%, and +118% respectively), suggesting a possible induction of glucuronidation processes by quercetin. This work allowed the characterization of actual phenolic metabolites generated in vivo following a phenolic intake, the analysis of their kinetics and suggested a possible synergistic activity of phenolic compounds for improving bioavailability.

引用

页码：3244 / 3256

页数：13

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