Selection of mRNA markers for detection of lymph node micrometastases in breast cancer patients

被引：5

作者：

Ooka, M ^{[1
]}

Sakita, I ^{[1
]}

Fujiwara, Y ^{[1
]}

Tamaki, Y ^{[1
]}

Yamamoto, H ^{[1
]}

Aihara, T ^{[1
]}

Miyazaki, M ^{[1
]}

Kadota, M ^{[1
]}

Masuda, N ^{[1
]}

Sugita, Y ^{[1
]}

Iwao, K ^{[1
]}

Monden, M ^{[1
]}

机构：

[1] Osaka Univ, Sch Med, Dept Surg 2, Suita, Osaka 5650871, Japan

来源：

ONCOLOGY REPORTS | 2000年 / 7卷 / 03期

关键词：

breast cancer; lymph node; RT-PCR; micrometastases;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The aim of this study was to search for specific and sensitive mRNA markers or a combination of markers for RT-PCR detection of micrometastases in axillary lymph nodes (LNs) from patients with breast cancer. LNs (n=177) from 17 patients were examined with Cytokeratin20 (CK20), melanoma-associated genes (MAGE1, MAGE3), carcino-embryonic antigen (CEA), prostate-specific antigen (PSA), mammaglobin (MGB1) and mammaglobin B (MGB2) as molecular markers. CK20, MAGE1 and MAGE3 were slightly positive in primary tumors and CEA, PSA, MGB1 and MGB2 were highly positive. MGB1 and MGB2 were 100% positive in HE-positive LNs while CEA and PSA were only 35.7% and 57.1% positive. MGB1 and MGB2 were also 30.1% and 17.8% positive in HE-negative nodes. Thus, MGB1 and MGB2 are specific and a combination of the two should be useful for detection of micrometastases in axillary LNs of breast cancer patients.

引用

页码：561 / 566

页数：6

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