Dual mechanism for cAMP-dependent modulation of Ca2+ signalling in articular chondrocytes

被引:13
作者
DAndrea, P
Paschini, V
Vittur, F
机构
[1] Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, 34127 Trieste
关键词
D O I
10.1042/bj3180569
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The ability of cAMP to modulate the actions of Ca2+-mobilizing agonists was studied in single Fura-2-loaded pig articular chondrocytes in primary culture. Forskolin and 8-Br-cAMP increased both the frequency and amplitude of Ca2+ oscillations induced by ATP, and, in unstimulated cells, induced single Ca2+ transients or even Ca2+ oscillations. The cAMP-dependent protein kinase inhibitor H89 totally prevented the effect of cAMP-elevating agents on Ca2+ signalling. Forskolin and 8-Br-cAMP promptly increased the rate of Mn2+ quenching, when administered in the presence of ATP, suggesting a potentiation of receptor-mediated Ca2+ influx. In Ca2+-free medium, ATP-induced Ca2+ oscillations decreased and stopped after a few cycles: subsequent ATP additions temporarily resumed the activity, an effect that could be mimicked by forskolin. The same agent induced single Ca2+ transients in 42%, of the cell population maintained in Ca2+-free medium. Thapsigargin prevented Ca2+ responses to both ATP and forskolin. The results indicate a dual mechanism for cAMP-induced potentiation of Ca2+ signalling in articular chondrocytes: an increase of receptor-mediated Ca2+ influx and a positive modulation of intracellular Ca2+ release.
引用
收藏
页码:569 / 573
页数:5
相关论文
共 27 条
[1]   CYCLIC AMP-INCREASING AGENTS INTERFERE WITH CHEMOATTRACTANT INDUCED RESPIRATORY BURST IN NEUTROPHILS AS A RESULT OF THE INHIBITION OF PHOSPHATIDYLINOSITOL 3-KINASE RATHER THAN RECEPTOR-OPERATED CA2+ INFLUX [J].
AHMED, MU ;
HAZEKI, K ;
HAZEKI, O ;
KATADA, T ;
UI, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (40) :23816-23822
[2]   INOSITOL TRISPHOSPHATE AND CALCIUM SIGNALING [J].
BERRIDGE, MJ .
NATURE, 1993, 361 (6410) :315-325
[3]   BELL-SHAPED CALCIUM-RESPONSE CURVES OF INS(1,4,5)P3-GATED AND CALCIUM-GATED CHANNELS FROM ENDOPLASMIC-RETICULUM OF CEREBELLUM [J].
BEZPROZVANNY, I ;
WATRAS, J ;
EHRLICH, BE .
NATURE, 1991, 351 (6329) :751-754
[4]   CYCLIC-NUCLEOTIDES AND INTRACELLULAR-CALCIUM HOMEOSTASIS IN HUMAN PLATELETS [J].
BRUNE, B ;
ULLRICH, V .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 207 (02) :607-613
[5]  
BURGESS GM, 1991, J BIOL CHEM, V266, P4772
[6]   EVIDENCE FOR THE PRESENCE OF P2-PURINOCEPTORS AT THE SURFACE OF HUMAN ARTICULAR CHONDROCYTES IN MONOLAYER-CULTURE [J].
CASWELL, AM ;
LEONG, WS ;
RUSSELL, RGG .
BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1074 (01) :151-158
[7]  
CHIJIWA T, 1990, J BIOL CHEM, V265, P5267
[8]   ADENYLYL CYCLASES AND THE INTERACTION BETWEEN CALCIUM AND CAMP SIGNALING [J].
COOPER, DMF ;
MONS, N ;
KARPEN, JW .
NATURE, 1995, 374 (6521) :421-424
[9]   SPATIAL AND TEMPORAL CA2+ SIGNALING IN ARTICULAR CHONDROCYTES [J].
DANDREA, P ;
VITTUR, F .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 215 (01) :129-135
[10]  
DANDREA P, 1996, J BONE MINER RES, V11, P939