Potential involvement of the IL-33-ST2 axis in the pathogenesis of primary Sjogren's syndrome

被引:63
作者
Awada, Ahmad [1 ,2 ]
Nicaise, Charles [3 ,4 ]
Ena, Sabrina [5 ]
Schandene, Liliane [6 ]
Rasschaert, Joanne [2 ]
Popescu, Iuliana [7 ]
Gangji, Valerie [1 ,2 ]
Soyfoo, Muhammad S. [1 ,2 ]
机构
[1] Univ Libre Bruxelles, Dept Rheumatol & Phys Med, Hop Erasme, B-1070 Brussels, Belgium
[2] Univ Libre Bruxelles, Lab Bone & Metab Biochem, B-1070 Brussels, Belgium
[3] Univ Libre Bruxelles, Dept Pathol, Hop Erasme, B-1070 Brussels, Belgium
[4] DIAPATH Ctr Microscopy & Mol Imaging CMMI, Gosselies, Belgium
[5] Univ Libre Bruxelles, Neurophysiol Lab, B-1070 Brussels, Belgium
[6] Univ Libre Bruxelles, Dept Clin Immunol, Hop Erasme, B-1070 Brussels, Belgium
[7] Univ Libre Bruxelles, Lab Gen Histol Neuroanat & Neuropathol, B-1070 Brussels, Belgium
关键词
INFLAMMATION; CYTOKINES; CELLS; IL-1; ST2;
D O I
10.1136/annrheumdis-2012-203187
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objectives To investigate the role of the interleukin (IL)-33-ST2 axis in the pathophysiology of primary Sjogren's syndrome (pSS). Methods Serum levels of IL-33 and sST2 were determined by ELISA. The expression of IL-33 and ST2 was investigated in salivary glands (SG) by immunohistochemistry. PBMC were isolated and stimulated with IL-33, IL-12 and IL-23 and the cytokine profile response was examined by flow cytometry. Intracellular cytokine detection of IFN gamma and IL-17 was performed by flow cytometry. Results Serum IL-33 and sST2 levels were increased in pSS patients compared with controls and patients with systemic lupus erythematosus. Expression of IL-33 was upregulated in SG with Chisholm scores of 2 and 3 of pSS patients but comparable with controls for SG with Chisholm score of 4. ST2 expression in SG was downregulated in pSS patients. IL-33 at different concentrations did not increase the secretion of pro-inflammatory cytokines but acted synergistically with IL-12 and IL-23 to promote IFN gamma production. NK and NKT cells were identified as main producers of IFN gamma in vitro and were found in SG of pSS patients. Conclusions IL-33 is released in pSS, and acts with IL-12 and IL-23 to favour the secretion of IFN gamma by NK and NKT cells.
引用
收藏
页码:1259 / 1263
页数:5
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