The importance of an endotoxin-free environment during the production of nanoparticles used in medical applications

被引：187

作者：

Vallhov, Helen ^{[1
]}

Qin, Jian

Johansson, Sara M.

Ahlborg, Niklas

Muhammed, Mamoun A.

Scheynius, Annika

Gabrielsson, Susanne

机构：

[1] Karolinska Inst, Dept Med, Clin Allergy Res Unit, S-17176 Stockholm, Sweden

[2] Univ Hosp, S-17176 Stockholm, Sweden

[3] Stockholm Univ, Dept Immunol, S-10691 Stockholm, Sweden

[4] Royal Inst Technol, Mat Chem Div, S-10044 Stockholm, Sweden

来源：

NANO LETTERS | 2006年 / 6卷 / 08期

关键词：

HUMAN DENDRITIC CELLS; GOLD; CONTAMINATION; MATURATION; PARTICLES; INNATE; METAL; SIZE;

D O I：

10.1021/nl060860z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We investigated the effect of spherical gold nanoparticles on immature dendritic cells (DCs). Conventionally produced nanoparticles had a maturating effect on the DCs-a result of lipopolysaccharide (LPS) contamination. By modification of the production process, low-LPS particles were obtained, which had practically no effect on phenotypic maturation or cytokine production of the DCs. Our findings emphasize the importance of high purity in the production of nanoparticles, since possible contaminants may interfere with the assessment of biological/medical effects. They also highlight that nanoparticles can function as carriers of immune modulating contaminants.

引用

页码：1682 / 1686

页数：5

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