A microfluidic cartridge to prepare spores for PCR analysis

被引：56

作者：

Belgrader, P ^{[1
]}

Okuzumi, M ^{[1
]}

Pourahmadi, F ^{[1
]}

Borkholder, DA ^{[1
]}

Northrup, MA ^{[1
]}

机构：

[1] Cepheid, Sunnyvale, CA 94089 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2000年 / 14卷 / 10-11期

关键词：

cartridge system; Bacillus spores; PCR;

D O I：

10.1016/S0956-5663(99)00060-3

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A prototype cartridge system is described that rapidly disrupts Bacillus spores by sonication, adds PCR reagent to the disrupted spores. and dispenses the mixture into a PCR tube. The total time to automatically process the spores in the cartridge and then detect the spore DNA by real-time PCR was 20 min. (C) 2000 Elsevier Science S.A. All rights reserved.

引用

收藏

页码：849 / 852

页数：4

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