IMPROVED METHODS FOR THE DETECTION OF BACILLUS-ANTHRACIS SPORES BY THE POLYMERASE CHAIN-REACTION

被引：28

作者：

JOHNS, M ^{[1
]}

HARRINGTON, L ^{[1
]}

TITBALL, RW ^{[1
]}

LESLIE, DL ^{[1
]}

机构：

[1] CHEM & BIOL DEF ESTAB,SALISBURY SP4 0JQ,WILTS,ENGLAND

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1994年 / 18卷 / 04期

关键词：

D O I：

10.1111/j.1472-765X.1994.tb00856.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Polymerase chain reactions (PCRs) for the capsule and oedema factor genes of Bacillus anthracis were used to assess methods for detecting B. anthracis spores. Untreated spore preparations were found to contain significant amounts of extracellular template DNA which probably accounted for observed amplification from these preparations without spore lysis. Germination of spores with suitable media allowed the detection of less than 10 spores in a PCR test. Mechanical disruption of spores with glass or zirconia beads yielded similar results to germination but in a much shorter time. The techniques described should improve the detection by PCR of B. anthracis and other sporulating bacteria.

引用

页码：236 / 238

页数：3

共 7 条

[1] DETECTION OF SPORES OF BACILLUS-ANTHRACIS USING THE POLYMERASE CHAIN-REACTION [J].