DETECTION OF SPORES OF BACILLUS-ANTHRACIS USING THE POLYMERASE CHAIN-REACTION

被引:21
作者
CARL, M
HAWKINS, R
COULSON, N
LOWE, J
ROBERTSON, DL
NELSON, WM
TITBALL, RW
WOODY, JN
机构
[1] UNIFORMED SERV UNIV HLTH SCI,BETHESDA,MD 20814
[2] USA,MED RES INST INFECT DIS,FREDERICK,MD 21701
[3] CHEM & BIOL DEF ESTAB,SALISBURY,ENGLAND
[4] BRIGHAM YOUNG UNIV,PROVO,UT 84602
关键词
D O I
10.1093/infdis/165.6.1145
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The polymerase chain reaction (PCR) was used to identify spores of Bacillus anthracis. By using an assay capable of amplifying a 1247-bp fragment from the gene that encodes the edema factor of B. anthracis, as few as 1O(3) copies of a plasmid containing the edema factor gene and as few as 2 x 10(4) spores were detected. Subjecting the product of this PCR to a second PCR designed to amplify a 208-bp fragment nested within the 1247-bp product improved detection to a single plasmid copy per PCR and to two spores of B. anthracis per PCR.
引用
收藏
页码:1145 / 1148
页数:4
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