DETECTION OF SPORES OF BACILLUS-ANTHRACIS USING THE POLYMERASE CHAIN-REACTION

被引：21

作者：

CARL, M

HAWKINS, R

COULSON, N

LOWE, J

ROBERTSON, DL

NELSON, WM

TITBALL, RW

WOODY, JN

机构：

[1] UNIFORMED SERV UNIV HLTH SCI,BETHESDA,MD 20814

[2] USA,MED RES INST INFECT DIS,FREDERICK,MD 21701

[3] CHEM & BIOL DEF ESTAB,SALISBURY,ENGLAND

[4] BRIGHAM YOUNG UNIV,PROVO,UT 84602

来源：

JOURNAL OF INFECTIOUS DISEASES | 1992年 / 165卷 / 06期

关键词：

D O I：

10.1093/infdis/165.6.1145

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The polymerase chain reaction (PCR) was used to identify spores of Bacillus anthracis. By using an assay capable of amplifying a 1247-bp fragment from the gene that encodes the edema factor of B. anthracis, as few as 1O(3) copies of a plasmid containing the edema factor gene and as few as 2 x 10(4) spores were detected. Subjecting the product of this PCR to a second PCR designed to amplify a 208-bp fragment nested within the 1247-bp product improved detection to a single plasmid copy per PCR and to two spores of B. anthracis per PCR.

引用

页码：1145 / 1148

页数：4