An integrated physical map for the short arm of human chromosome 5

被引：5

作者：

Peterson, ET

Sutherland, R

Robinson, DL

Chasteen, L

Gersh, M

Overhauser, J

Deaven, LL

Moyzis, RK

Grady, DL ^{[1
]}

机构：

[1] Los Alamos Natl Lab, Div Life Sci, Los Alamos, NM 87545 USA

[2] Los Alamos Natl Lab, Ctr Human Genome Studies, Los Alamos, NM 87545 USA

[3] Los Alamos Natl Lab, Software Design & Dev Grp, Los Alamos, NM 87545 USA

[4] Thomas Jefferson Univ, Dept Mol Pharmacol & Biochem, Philadelphia, PA 19107 USA

[5] Univ Calif Irvine, Coll Med, Dept Biol Chem, Irvine, CA 92697 USA

来源：

GENOME RESEARCH | 1999年 / 9卷 / 12期

关键词：

D O I：

10.1101/gr.9.12.1250

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The short arm of human chromosome 5 contains similar to 48 Mb of DNA and comprises 1.5% of the genome. We have constructed a mega-YAC/ STS map of this region that includes 436 YACs anchored by 216 STSs. By combining and integrating our map with the 5p maps of other groups using the same recombinant DNA library, a comprehensive map was constructed that includes 552 YACs and 504 markers. The YAC map covers >94% of 5p in four YAC contigs, bridges the centromere, and includes an additional 5 Mb of 5q DNA. The average marker density is 95 kb. This integrated 5p map will serve as a resource for the continuing Localization of genes on the short arm of human chromosome 5 and as a framework for both generating and aligning the DNA sequence of this region.

引用

页码：1250 / 1267

页数：18

共 53 条

[11]

COLLINS JE, 1995, NATURE, V377, P367

[12] A physical map of 30,000 human genes [J].

Deloukas, P ;

Schuler, GD ;

Gyapay, G ;

Beasley, EM ;

Soderlund, C ;

Rodriguez-Tomé, P ;

Hui, L ;

Matise, TC ;

McKusick, KB ;

Beckmann, JS ;

Bentolila, S ;

Bihoreau, MT ;

Birren, BB ;

Browne, J ;

Butler, A ;

Castle, AB ;

Chiannilkulchai, N ;

Clee, C ;

Day, PJR ;

Dehejia, A ;

Dibling, T ;

Drouot, N ;

Duprat, S ;

Fizames, C ;

Fox, S ;

Gelling, S ;

Green, L ;

Harrison, P ;

Hocking, R ;

Holloway, E ;

Hunt, S ;

Keil, S ;

Lijnzaad, P ;

Louis-Dit-Sully, C ;

Ma, J ;

Mendis, A ;

Miller, J ;

Morissette, J ;

Muselet, D ;

Nusbaum, HC ;

Peck, A ;

Rozen, S ;

Simon, D ;

Slonim, DK ;

Staples, R ;

Stein, LD ;

Stewart, EA ;

Suchard, MA ;

Thangarajah, T ;

Vega-Czarny, N .

SCIENCE, 1998, 282 (5389) :744-746

[13] A comprehensive genetic map of the human genome based on 5,264 microsatellites [J].

Dib, C ;

Faure, S ;

Fizames, C ;

Samson, D ;

Drouot, N ;

Vignal, A ;

Millasseau, P ;

Marc, S ;

Hazan, J ;

Seboun, E ;

Lathrop, M ;

Gyapay, G ;

Morissette, J ;

Weissenbach, J .

NATURE, 1996, 380 (6570) :152-154

[14]

DOGGETT NA, 1995, NATURE, V377, P335

[15] Repetitive conundrums of centromere structure and function [J].

Eichler, EE .

HUMAN MOLECULAR GENETICS, 1999, 8 (02) :151-155

[16] THE HUMAN Y-CHROMOSOME - OVERLAPPING DNA CLONES SPANNING THE EUCHROMATIC REGION [J].

FOOTE, S ;

VOLLRATH, D ;

HILTON, A ;

PAGE, DC .

SCIENCE, 1992, 258 (5079) :60-66

[17]

GEMMILL RM, 1995, NATURE, V377, P299

[18] PHYSICAL MAPPING OF GENETIC-MARKERS ON THE SHORT ARM OF CHROMOSOME-5 [J].

GERSH, M ;

GOODART, SA ;

OVERHAUSER, J .

GENOMICS, 1994, 24 (03) :577-579

[19] Development of diagnostic tools for the analysis of 5p deletions using interphase FISH [J].

Gersh, M ;

Grady, D ;

Rojas, K ;

Lovett, M ;

Moyzis, R ;

Overhauser, J .

CYTOGENETICS AND CELL GENETICS, 1997, 77 (3-4) :246-251

[20]

GERSH M, 1995, AM J HUM GENET, V56, P1404

← 1 2 3 4 5 6 →