Interpretation of Negative Molecular Test Results in Patients With Suspected or Confirmed Ebola Virus Disease: Report of Two Cases

被引：9

作者：

Edwards, Jeffrey K. ^{[1
,2
]}

Kleine, Christian ^{[1
,3
]}

Munster, Vincent ^{[4
]}

Giuliani, Ruggero ^{[1
]}

Massaquoi, Moses ^{[5
]}

Sprecher, Armand ^{[1
]}

Chertow, Daniel S. ^{[1
,6
]}

机构：

[1] Medecins Sans Frontieres, Operat Ctr Brussels, B-1050 Brussels, Belgium

[2] Johns Hopkins Univ, Sch Publ Hlth, Dept Int Hlth, Baltimore, MD USA

[3] JW Goethe Univ Hosp, Dept Infect Dis & Trop Med, Frankfurt, Germany

[4] NIAID, Virol Lab, NIH, Rocky Mt Labs, Hamilton, MT USA

[5] Clinton Hlth Initiat, Monrovia, CA, Liberia

[6] NIH, Dept Crit Care Med, Ctr Clin, Bethesda, MD 20892 USA

来源：

OPEN FORUM INFECTIOUS DISEASES | 2015年 / 2卷 / 04期

基金：

美国国家卫生研究院;

关键词：

Ebola; hemorrhagic fever; Liberia; PCR; West Africa; CASE-DEFINITION; WEST-AFRICA; OUTBREAK;

D O I：

10.1093/ofid/ofv137

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

071005 [微生物学]; 100108 [医学免疫学];

摘要：

Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) is the most sensitive quantitative diagnostic assay for detection of Ebola virus in multiple body fluids. Despite the strengths of this assay, we present 2 cases of Ebola virus disease (EVD) and highlight the potential for false-negative results during the early and late stages of EVD. The first case emphasizes the low negative-predictive value of qR-TPCR during incubation and the early febrile stage of EVD, and the second case emphasizes the potential for false-negative results during recovery and late neurologic complications of EVD. Careful interpretation of test results are needed to guide difficult admission and discharge decisions in suspected or confirmed EVD.

引用

页码：1 / 4

页数：4

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