In vitro characterization of 3D printed scaffolds aimed at bone tissue regeneration

被引:68
作者
Boga, Joao C. [1 ]
Miguel, Sonia P. [1 ]
de Melo-Diogo, Duarte [1 ]
Mendonca, Antonio G. [1 ,2 ]
Louro, Ricardo O. [3 ]
Correia, Ilidio J. [1 ,4 ]
机构
[1] Univ Beira Interior, CICS, Av Infante D Henrique, P-6200506 Covilha, Portugal
[2] Univ Beira Interior, Dept Quim, R Marques dAvila & Bolama, P-6201001 Covilha, Portugal
[3] Univ Nova Lisboa, ITQB Inst Tecnol Quim & Biol Antonio Xavier, P-2780157 Oeiras, Portugal
[4] Univ Coimbra, CIEPQPF Dept Engn Quim, Rua Silvio Lima, P-3030790 Coimbra, Portugal
关键词
Bone tissue engineering; Rapid prototyping; 3D printing; Cylindrical hybrid scaffolds; Graphene oxide; BETA-TRICALCIUM PHOSPHATE; MESENCHYMAL STEM-CELLS; FUNCTIONALIZED GRAPHENE OXIDE; OSTEOGENIC DIFFERENTIATION; COMPOSITE SCAFFOLDS; SURFACE WETTABILITY; PHYSICAL-PROPERTIES; PROTEIN ADSORPTION; ALGINATE; HYDROXYAPATITE;
D O I
10.1016/j.colsurfb.2018.02.038
中图分类号
Q6 [生物物理学];
学科分类号
071011 [生物物理学];
摘要
The incidence of fractures and bone-related diseases like osteoporosis has been increasing due to aging of the world's population. Up to now, grafts and titanium implants have been the principal therapeutic approaches used for bone repair/regeneration. However, these types of treatment have several shortcomings, like limited availability, risk of donor-to-recipient infection and tissue morbidity. To overcome these handicaps, new 3D templates, capable of replicating the features of the native tissue, are currently being developed by researchers from the area of tissue engineering. These 3D constructs are able to provide a temporary matrix on which host cells can adhere, proliferate and differentiate. Herein, 3D cylindrical scaffolds were designed to mimic the natural architecture of hollow bones, and to allow nutrient exchange and bone neovascularization. 3D scaffolds were produced with tricalcium phosphate (TCP)/alginic acid (AA) using a Fab@home 3D printer. Furthermore, graphene oxide (GO) was incorporated into the structure of some scaffolds to further enhance their mechanical properties. The results revealed that the scaffolds incorporating GO displayed greater porosity, without impairing their mechanical properties. These scaffolds also presented a controlled swelling profile, enhanced biomineralization capacity and were able to increase the Alkaline Phosphatase (ALP) activity. Such characteristics make TCP/AA scaffolds functionalized with GO promising 3D constructs for bone tissue engineering applications. (C) 2018 Elsevier B.V. All rights reserved.
引用
收藏
页码:207 / 218
页数:12
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