Bioimprinted QCM sensors for virus detection - screening of plant sap

被引：130

作者：

Dickert, FL

Hayden, O

Bindeus, R

Mann, KJ

Blaas, D

Waigmann, E

机构：

[1] Univ Vienna, Inst Analyt Chem, A-1090 Vienna, Austria

[2] Univ Vienna, Inst Med Biochem, A-1030 Vienna, Austria

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2004年 / 378卷 / 08期

基金：

奥地利科学基金会;

关键词：

tobacco mosaic virus; quartz-crystal microbalance; molecular imprinting; atomic force microscopy (AFM);

D O I：

10.1007/s00216-004-2521-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Surface imprinting techniques on polymer-coated quartz-crystal microbalances (QCM) have been used to detect tobacco mosaic viruses (TMV) in aqueous media. Molecularly imprinted polymers (MIP), tailor-made by self organisation of monomers around a template (TMV), were generated directly on the gold electrodes. Imprinted trenches on the polymer surface mimicking the shape and surface functionality of the virus serve as recognition sites for re-adsorption after washing out of the template. The sensors are applicable to TMV detection ranging from 100 ng mL(-1) to 1 mg mL(-1) within minutes. Furthermore, direct measurements without time-consuming sample preparation are possible in complex matrices such as tobacco plant sap.

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收藏

页码：1929 / 1934

页数：6

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