The Arabidopsis RING Finger E3 Ligase RHA2a Is a Novel Positive Regulator of Abscisic Acid Signaling during Seed Germination and Early Seedling Development

被引：150

作者：

Bu, Qingyun ^{[1
]}

Li, Hongmei ^{[1
,2
]}

Zhao, Qingzhen ^{[1
,2
]}

Jiang, Hongling ^{[1
]}

Zhai, Qingzhe ^{[1
,2
]}

Zhang, Jie ^{[1
,2
]}

Wu, Xiaoyan ^{[1
]}

Sun, Jiaqiang ^{[1
]}

Xie, Qi ^{[1
]}

Wang, Daowen ^{[3
]}

Li, Chuanyou ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, State Key Lab Plant Genom, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

[3] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China

来源：

PLANT PHYSIOLOGY | 2009年 / 150卷 / 01期

基金：

中国国家自然科学基金;

关键词：

NAC TRANSCRIPTION FACTORS; NEGATIVE REGULATOR; G-PROTEIN; FUNCTIONAL-ANALYSIS; GENE; UBIQUITIN; STRESS; DEGRADATION; GROWTH; TRANSDUCTION;

D O I：

10.1104/pp.109.135269

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

The phytohormone abscisic acid (ABA) is well known for its regulatory roles in integrating environmental constraints with the developmental programs of plants. Here, we characterize the biological function of the Arabidopsis (Arabidopsis thaliana) RING-H2 protein RHA2a in ABA signaling. The rha2a mutant is less sensitive to ABA than the wild type during seed germination and early seedling development, whereas transgenic plants overexpressing RHA2a are hypersensitive, indicating that RHA2a positively regulates ABA-mediated control of seed germination and early seedling development. Double mutant analyses of rha2a with several known ABA-insensitive mutants suggest that the action of RHA2a in ABA signaling is independent of that of the transcription factors ABI3, ABI4, and ABI5. We provide evidence showing that RHA2a also positively regulates plant responses to salt and osmotic stresses during seed germination and early seedling development. RHA2a is a functional E3 ubiquitin ligase, and its conserved RING domain is likely important for the biological function of RHA2a in ABA signaling. Together, these results suggest that the E3 ligase RHA2a is an important regulator of ABA signaling during seed germination and early seedling development.

引用

页码：463 / 481

页数：19

共 62 条

[1]

Arabidopsis abi1-1 and abi2-1 phosphatase mutations reduce abscisic acid-induced cytoplasmic calcium rises in guard cells [J].