Finding the fifth base: Genome-wide sequencing of cytosine methylation

被引：245

作者：

Lister, Ryan ^{[1
,2
]}

Ecker, Joseph R. ^{[1
,2
]}

机构：

[1] Salk Inst Biol Studies, Genome Anal Lab, La Jolla, CA 92037 USA

[2] Salk Inst Biol Studies, Plant Biol Lab, La Jolla, CA 92037 USA

来源：

GENOME RESEARCH | 2009年 / 19卷 / 06期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

DIRECTED DNA METHYLATION; DE-NOVO METHYLATION; ARABIDOPSIS-THALIANA; EPIGENETIC INHERITANCE; MAMMALIAN DEVELOPMENT; TILING MICROARRAYS; PROMOTER REGION; CPG METHYLATION; SP1; BINDING; HUMAN-CELLS;

D O I：

10.1101/gr.083451.108

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Complete sequences of myriad eukaryotic genomes, including several human genomes, are now available, and recent dramatic developments in DNA sequencing technology are opening the floodgates to vast volumes of sequence data. Yet, despite knowing for several decades that a significant proportion of cytosines in the genomes of plants and animals are present in the form of methylcytosine, until very recently the precise locations of these modified bases have never been accurately mapped throughout a eukaryotic genome. Advanced "next-generation'' DNA sequencing technologies are now enabling the global mapping of this epigenetic modification at single-base resolution, providing new insights into the regulation and dynamics of DNA methylation in genomes.

引用

页码：959 / 966

页数：8

共 72 条

[1]

Absalan Farnaz, 2007, V396, P315

[2] Direct selection of human genomic loci by microarray hybridization [J].

Albert, Thomas J. ;

Molla, Michael N. ;

Muzny, Donna M. ;

Nazareth, Lynne ;

Wheeler, David ;

Song, Xingzhi ;

Richmond, Todd A. ;

Middle, Chris M. ;

Rodesch, Matthew J. ;

Packard, Charles J. ;

Weinstock, George M. ;

Gibbs, Richard A. .

NATURE METHODS, 2007, 4 (11) :903-905

[3] Targeted and genome-scale strategies reveal gene-body methylation signatures in human cells [J].

Ball, Madeleine P. ;

Li, Jin Billy ;

Gao, Yuan ;

Lee, Je-Hyuk ;

LeProust, Emily M. ;

Park, In-Hyun ;

Xie, Bin ;

Daley, George Q. ;

Church, George M. .

NATURE BIOTECHNOLOGY, 2009, 27 (04) :361-368

[4] Arabidopsis cmt3 chromomethylase mutations block non-CG methylation and silencing of an endogenous gene [J].

Bartee, L ;

Malagnac, F ;

Bender, J .

GENES & DEVELOPMENT, 2001, 15 (14) :1753-1758

[5] Direct genomic selection [J].

Bashiardes, S ;

Veile, R ;

Helms, C ;

Mardis, ER ;

Bowcock, AM ;

Lovett, M .

NATURE METHODS, 2005, 2 (01) :63-69

[6] The methylome: approaches for global DNA methylation profiling [J].

Beck, Stephan ;

Rakyan, Vardhman K. .

TRENDS IN GENETICS, 2008, 24 (05) :231-237

[7] Methylation of a CTCF-dependent boundary controls imprinted expression of the Igf2 gene [J].

Bell, AC ;

Felsenfeld, G .

NATURE, 2000, 405 (6785) :482-485

[8] The mammalian epigenome [J].

Bernstein, Bradley E. ;

Meissner, Alexander ;

Lander, Eric S. .

CELL, 2007, 128 (04) :669-681

[9] The DNA methyltransferases of mammals [J].

Bestor, TH .

HUMAN MOLECULAR GENETICS, 2000, 9 (16) :2395-2402

[10] Human embryonic stem cells have a unique epigenetic signature [J].

Bibikova, Marina ;

Chudin, Eugene ;

Wu, Bonnie ;

Zhou, Lixin ;

Garcia, Eliza Wickham ;

Liu, Ying ;

Shin, Soojung ;

Plaia, Todd W. ;

Auerbach, Jonathan M. ;

Arking, Dan E. ;

Gonzalez, Rodolfo ;

Crook, Jeremy ;

Davidson, Bruce ;

Schulz, Thomas C. ;

Robins, Allan ;

Khanna, Aparna ;

Sartipy, Peter ;

Hyllner, Johan ;

Vanguri, Padmavathy ;

Savant-Bhonsale, Smita ;

Smith, Alan K. ;

Chakravarti, Aravinda ;

Maitra, Anirban ;

Rao, Mahendra ;

Barker, David L. ;

Loring, Jeanne F. ;

Fan, Jian-Bing .

GENOME RESEARCH, 2006, 16 (09) :1075-1083

← 1 2 3 4 5 6 7 8 →