Biology and Applications of CRISPR Systems: Harnessing Nature's Toolbox for Genome Engineering

被引：747

作者：

Wright, Addison V. ^{[1
]}

Nunez, James K. ^{[1
]}

Doudna, Jennifer A. ^{[1
,2
,3
,4
,5
,6
]}

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Howard Hughes Med Inst HHMI, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[4] Univ Calif Berkeley, Ctr RNA Syst Biol, Berkeley, CA 94720 USA

[5] Univ Calif Berkeley, Innovat Genom Initiat, Berkeley, CA 94720 USA

[6] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USA

来源：

CELL | 2016年 / 164卷 / 1-2期

基金：

美国国家科学基金会;

关键词：

GUIDED SURVEILLANCE COMPLEX; IN-VITRO RECONSTITUTION; PROCESSES PRE-CRRNA; R-LOOP FORMATION; CRYSTAL-STRUCTURE; ESCHERICHIA-COLI; CAS SYSTEM; SPACER ACQUISITION; DNA CLEAVAGE; FOREIGN DNA;

D O I：

10.1016/j.cell.2015.12.035

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacteria and archaea possess a range of defense mechanisms to combat plasmids and viral infections. Unique among these are the CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR associated) systems, which provide adaptive immunity against foreign nucleic acids. CRISPR systems function by acquiring genetic records of invaders to facilitate robust interference upon reinfection. In this Review, we discuss recent advances in understanding the diverse mechanisms by which Cas proteins respond to foreign nucleic acids and how these systems have been harnessed for precision genome manipulation in a wide array of organisms.

引用

页码：29 / 44

页数：16

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