Characterization of proanthocyanidins from grape seeds

被引：194

作者：

Gabetta, B ^{[1
]}

Fuzzati, N ^{[1
]}

Griffini, A ^{[1
]}

Lolla, E ^{[1
]}

Pace, R ^{[1
]}

Ruffilli, T ^{[1
]}

Peterlongo, F ^{[1
]}

机构：

[1] Indena SPA, Lab Ric & Sviluppo, I-20090 Milan, Italy

来源：

FITOTERAPIA | 2000年 / 71卷 / 02期

关键词：

Vitis vinifera; grape seeds; proanthocyanidins; GPC; HPLC TSP-MS; ESI-MS;

D O I：

10.1016/S0367-326X(99)00161-6

中图分类号：

R914 [药物化学];

学科分类号：

100701 [药物化学];

摘要：

Leucoselect(TM) (grape seed selected proanthocyanidins) was analyzed. HPLC thermospray mass spectrometry (TSP-MS) allowed the detection of monomeric flavan-3-ols and dimeric proanthocyanidins. Fractionation over Sephadex(R) LH-20 resin and analysis of the isolated fractions by gel permeation chromatography (GPC) and electrospray mass spectrometry (ESI-MS) led to the complete characterization of the proanthocyanidin constituents of Leucoselect(TM). The analysis revealed the presence of approximately 15% of (+)-catechin (1) and (-)-epicatechin (2), 80% of (-)-epicatechin 3-O-gallate (3), dimers, trimers, tetramers and their gallates and 5% of pentamers, hexamers, heptamers and their gallates. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：162 / 175

页数：14

共 34 条

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