Use of dynamic combinatorial chemistry for the identification of covalently appended residues that stabilize oligonucleotide complexes

被引：37

作者：

Bugaut, A

Toulmé, JJ

Rayner, B

机构：

[1] Univ Bordeaux 2, INSERM, U386, F-330763 Bordeaux, France

[2] IECB, F-33607 Pessac, France

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 24期

关键词：

dynamic combinatorial chemistry; molecular recognition; nucleic acids; oligonucleoticles; RNA structures;

D O I：

10.1002/anie.200454041

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Amplification with kissing: Rapid identification of covalently appended residues that stabilize oligonucleotide complexes has been achieved by using a dynamic combinatorial approach that leads to amplification of the best ligand (see schematic representation). Both a DNA duplex and an RNA loop-loop (kissing) complex were investigated. Melting-temperature experiments confirmed that the amplified species stabilize the complexes.

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收藏

页码：3144 / 3147

页数：4

相关论文

共 22 条

[1] Selection of modified oligonucleotides with increased target affinity via MALDI-monitored nuclease survival assays [J].

Altman, RK ;

Schwope, I ;

Sarracino, DA ;

Tetzlaff, CN ;

Bleczinski, CF ;

Richert, C .

JOURNAL OF COMBINATORIAL CHEMISTRY, 1999, 1 (06) :493-508

[2] Inhibition of HIV-1 Tat-dependent trans activation by steric block chimeric 2′-O-methyl/LNA oligoribonucleotides [J].

Arzumanov, A ;

Walsh, AP ;

Rajwanshi, VK ;

Kumar, R ;

Wengel, J ;

Gait, MJ .

BIOCHEMISTRY, 2001, 40 (48) :14645-14654

[3] OLIGONUCLEOTIDE DUPLEXES CONTAINING 2'-AMINO-2'-DEOXYCYTIDINES - THERMAL-STABILITY AND CHEMICAL-REACTIVITY [J].

AURUP, H ;

TUSCHL, T ;

BENSELER, F ;

LUDWIG, J ;

ECKSTEIN, F .

NUCLEIC ACIDS RESEARCH, 1994, 22 (01) :20-24

[4] Molecular dynamics reveals the stabilizing role of loop closing residues in kissing interactions:: comparison between TAR-TAR* and TAR-aptamer [J].

Beaurain, F ;

Di Primo, C ;

Toulmé, JJ ;

Laguerre, M .

NUCLEIC ACIDS RESEARCH, 2003, 31 (14) :4275-4284

[5] CYANOHYDRIDOBORATE ANION AS A SELECTIVE REDUCING AGENT [J].

BORCH, RF ;

BERNSTEIN, MD ;

DURST, HD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1971, 93 (12) :2897-+

[6]

DARFEUILLE F, 2002, THESIS U VICTOR SEGA

[7] In vitro selection identifies key determinants for loop-loop interactions:: RNA aptamers selective for the TAR RNA element of HIV-1 [J].

Ducongé, F ;

Toulmé, JJ .

RNA, 1999, 5 (12) :1605-1614

[8] TEMPLATE-DIRECTED SYNTHESIS - USE OF A REVERSIBLE-REACTION [J].

GOODWIN, JT ;

LYNN, DG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (23) :9197-9198

[9]

Huc I, 2001, COMB CHEM HIGH T SCR, V4, P53

[10] Virtual combinatorial libraries: Dynamic generation of molecular and supramolecular diversity by self-assembly [J].

Huc, I ;

Lehn, JM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (06) :2106-2110