Use of dynamic combinatorial chemistry for the identification of covalently appended residues that stabilize oligonucleotide complexes

被引：37

作者：

Bugaut, A

Toulmé, JJ

Rayner, B

机构：

[1] Univ Bordeaux 2, INSERM, U386, F-330763 Bordeaux, France

[2] IECB, F-33607 Pessac, France

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 24期

关键词：

dynamic combinatorial chemistry; molecular recognition; nucleic acids; oligonucleoticles; RNA structures;

D O I：

10.1002/anie.200454041

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Amplification with kissing: Rapid identification of covalently appended residues that stabilize oligonucleotide complexes has been achieved by using a dynamic combinatorial approach that leads to amplification of the best ligand (see schematic representation). Both a DNA duplex and an RNA loop-loop (kissing) complex were investigated. Melting-temperature experiments confirmed that the amplified species stabilize the complexes.

引用

页码：3144 / 3147

页数：4