Personal exposure to house dust mite allergen in bed: nasal air sampling and reservoir allergen levels

被引:28
作者
Gore, RB
Hadi, EA
Craven, M
Smillie, FI
O'Meara, TJ
Tovey, ER
Woodcock, A
Custovic, A
机构
[1] Wythenshawe Hosp, NW Lung Res Ctr, Manchester M23 9LT, Lancs, England
[2] Univ Sydney, Inst Resp Med, Sydney, NSW 2006, Australia
关键词
house dust mite; nasal air sampling; allergen exposure;
D O I
10.1046/j.1365-2222.2002.01403.x
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background Assessment of personal exposure to dust mite allergen has relied on proxy measures. Only recently has a means to directly measure inhaled allergen particle number become available (the intra-nasal air sampler). Objective To quantify inspired dust mite group 1 and group 2 allergen-bearing particles in bed in undisturbed conditions prior to sleep by nasal air sampling and to investigate the relationship between inhaled particles and reservoir allergen levels. Methods Twelve volunteers wore nasal samplers in bed for 6 evenings, nose-breathing in undisturbed conditions. Allergen-bearing particles ('halos') were detected by immunostaining for Der p 1, Der p 2, or Der p 1 and Der p 2 together, and counted by light microscopy. Count data were square root transformed for analysis of variance. Mattress dust samples were assayed for Der p 1 and Der p 2 concentrations. Results Square root detransformed mean particle counts per 30-min sample were: Der p 1, 4.22; Der p 2, 5.9 Der p 1 + Der p 2. 4.87 and for all samples, 5.01, with no difference between the groups. With replicate samples. halo number correlated significantly with mattress allergen concentrations (Der p 1 r = 0.80, P < 0.01 Der p 2 r = 0.68, P < 0.02). Conclusion Nasal air sampling can be used to quantify nocturnal Der p exposure in undisturbed conditions in an area with moderate exposure to mite allergen and can provide a direct measure of inhaled mite allergen. The choice of either Der p 1 or Der p 2 is appropriate for this purpose.
引用
收藏
页码:856 / 859
页数:4
相关论文
共 17 条
[1]  
ALTMAN DG, 1993, PRACTICAL STAT MED R, P143
[2]   Dust mite allergens are carried on not only large particles [J].
Custovic, A ;
Woodcock, H ;
Craven, M ;
Hassall, R ;
Hadley, E ;
Simpson, A ;
Woodcock, A .
PEDIATRIC ALLERGY AND IMMUNOLOGY, 1999, 10 (04) :258-260
[3]   Manchester Asthma and Allergy Study: Low-allergen environment can be achieved and maintained during pregnancy and in early life [J].
Custovic, A ;
Simpson, BM ;
Simpson, A ;
Hallam, C ;
Craven, M ;
Brutsche, M ;
Woodcock, A .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2000, 105 (02) :252-258
[4]   Mite allergen (Der p 1) is not only carried on mite feces [J].
De Lucca, S ;
Sporik, R ;
O'Meara, T ;
Tovey, ER .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1999, 103 (01) :174-175
[5]   AIRBORNE DUST MITE ALLERGENS - COMPARISON OF GROUP II ALLERGENS WITH GROUP-I MITE ALLERGEN AND CAT-ALLERGEN FEL D I [J].
DEBLAY, F ;
HEYMANN, PW ;
CHAPMAN, MD ;
PLATTSMILLS, TAE .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1991, 88 (06) :919-926
[6]   The nasal air sampler: a device for sampling inhaled aeroallergens [J].
Graham, JAH ;
Pavlicek, PK ;
Sercombe, JK ;
Xavier, ML ;
Tovey, ER .
ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, 2000, 84 (06) :599-604
[7]  
LUCZYNSKA CM, 1989, J IMMUNOL METHODS, V118, P27
[8]   Detection of inhaled cat allergen [J].
O'Meara, TJ ;
De Lucca, S ;
Sporik, R ;
Graham, A ;
Tovey, E .
LANCET, 1998, 351 (9114) :1488-1489
[9]   MONOCLONAL-ANTIBODIES TO GROUP-II DERMATOPHAGOIDES SPP ALLERGENS - MURINE IMMUNE-RESPONSE, EPITOPE ANALYSIS, AND DEVELOPMENT OF A 2-SITE ELISA [J].
OVSYANNIKOVA, IG ;
VAILES, LD ;
LI, Y ;
HEYMANN, PW ;
CHAPMAN, MD .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1994, 94 (03) :537-546
[10]  
Park GM, 2000, CLIN EXP ALLERGY, V30, P1293, DOI 10.1046/j.1365-2222.2000.00883.x