Development and validation of a quantitative method for the determination of 12 endocannabinoids and related compounds in human plasma using liquid chromatography-tandem mass spectrometry

被引：81

作者：

Balvers, Michiel G. J. ^{[1
,2
,3
]}

Verhoeckx, Kitty C. M. ^{[1
,2
]}

Witkamp, Renger F. ^{[1
,2
,3
]}

机构：

[1] TNO Qual Life, Dept Analyt Res, NL-3700 AJ Zeist, Netherlands

[2] TNO Qual Life, Dept Biosci, NL-3700 AJ Zeist, Netherlands

[3] Wageningen Univ, Div Human Nutr, NL-6700 EV Wageningen, Netherlands

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 14-15期

关键词：

Endocannabinoids; LC-MS/MS; Sample pre-treatment; Human plasma; FATTY-ACID AMIDES; CANNABINOID RECEPTOR; RAT-BRAIN; ENDOGENOUS CANNABINOIDS; ANANDAMIDE; PAIN; PALMITOYLETHANOLAMIDE; INFLAMMATION; DISORDERS; SYSTEM;

D O I：

10.1016/j.jchromb.2009.04.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and specific LC-MS/MS method for the quantification of the endocannabinoids and related structures anandamide, 2-arachidonoyl glycerol, 2-arachidonyl glycerol ether, O-arachidonoyl ethanolamide, dihomo-gamma-linolenoyl ethanolamide, docosatetraenoyl ethanolamide, N-arachidonoyl dopamine, N-arachidonyl glycine, N-oleoyl dopamine, oleoyl ethanolamide, palmitoyl ethanolamide, and stearoyl ethanolamide in human plasma was developed and validated. Compounds were extracted using acetonitrile followed by solid-phase extraction. Separation was performed on a Xterra C8 column using gradient elution coupled to a triple-quadrupole MS. LLOQ levels ranged from 0.02 to 1.75 mu g/mL, LODs ranged from 0.0002 to 0.1266 ng/mL, and accuracies were >80% (except stearoyl ethanolamide at lowest spike level) at all spike levels. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：1583 / 1590

页数：8

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