Promoter activity of the pER341-borne STPhsp in heterologous gene expression in Escherichia coli and Streptococcus thermophilus

被引：13

作者：

Somkuti, GA ^{[1
]}

Steinberg, DH ^{[1
]}

机构：

[1] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA

来源：

FEMS MICROBIOLOGY LETTERS | 1999年 / 179卷 / 02期

关键词：

plasmid-borne promoter; STPhsp promoter; pER341; S; thermophilus;

D O I：

10.1016/S0378-1097(99)00446-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A 138-bp EcoO109/HinfI fragment of Streptococcus thermophilus plasmid pER341 (2798 bp) including the promoter sequence of the heat stress protein gene hsp16.4 was tested in vector constructs for ability to activate the promoterless green fluorescent protein gene (gfp) from a jelly fish in Escherichia coli, S. thermophilus, and lactococci. STPhsp promoted gfp expression in transformed hosts as evidenced by the presence of green fluorescent (GFP(+)) colonies under UV illumination. The results confirmed the potential of STPhsp as a functional promoter in heterologous gene expression in dairy fermentation bacteria. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：431 / 436

页数：6

共 25 条

[1]

Ausubel FM, 1995, SHORT PROTOCOLS MOL

[2] ISOLATION AND CHARACTERIZATION OF PROMOTER REGIONS FROM STREPTOCOCCUS-THERMOPHILUS [J].

CONSTABLE, A ;

MOLLET, B .

FEMS MICROBIOLOGY LETTERS, 1994, 122 (1-2) :85-90

[3]

Maniatis T., 1982, MOL CLONING A LAB MA

[4]

MERCENIER A, 1990, FEMS MICROBIOL LETT, V87, P61, DOI 10.1111/j.1574-6968.1990.tb04879.x

[5] EXPRESSION OF A LACTOCOCCUS-LACTIS PHAGE RESISTANCE MECHANISM BY STREPTOCOCCUS-THERMOPHILUS [J].

MOINEAU, S ;

WALKER, SA ;

HOLLER, BJ ;

VEDAMUTHU, ER ;

VANDENBERGH, PA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (07) :2461-2466

[6] DIRECTED GENOMIC INTEGRATION, GENE REPLACEMENT, AND INTEGRATIVE GENE-EXPRESSION IN STREPTOCOCCUS-THERMOPHILUS [J].

MOLLET, B ;

KNOL, J ;

POOLMAN, B ;

MARCISET, O ;

DELLEY, M .

JOURNAL OF BACTERIOLOGY, 1993, 175 (14) :4315-4324

[7] ISOLATION AND CHARACTERIZATION OF CHROMOSOMAL PROMOTERS OF STREPTOCOCCUS-SALIVARIUS SUBSP THERMOPHILUS [J].

SLOS, P ;

BOURQUIN, JC ;

LEMOINE, Y ;

MERCENIER, A .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (05) :1333-1339

[8] Isolation and characterization of transcription signal sequences from Streptococcus thermophilus [J].

Solaiman, DKY ;

Somkuti, GA .

CURRENT MICROBIOLOGY, 1997, 34 (04) :216-219

[9]

SOLAIMAN DKY, 1995, APPL MICROBIOL BIOT, V43, P285

[10] Characterization of pER371-based Streptococcus thermophilus Escherichia coli shuttle vectors [J].

Solaiman, DKY ;

Somkuti, GA .

BIOTECHNOLOGY LETTERS, 1997, 19 (07) :595-598

← 1 2 3 →