Gold Nanoparticles Generated in Ethosome Bilayers, As Revealed by Cryo-Electron-Tomography

被引：25

作者：

de la Presa, Patricia ^{[1
]}

Rueda, Tatiana ^{[1
]}

del Puerto Morales, Maria ^{[2
]}

Javier Chichon, F. ^{[3
]}

Arranz, Rocio ^{[3
]}

Maria Valpuesta, Jose ^{[3
]}

Hernando, Antonio ^{[1
]}

机构：

[1] UCM, ADIF, CSIC, Inst Magnetismo Aplicado, Madrid 28230, Spain

[2] CSIC Cantoblanco, Inst Ciencia Mat Madrid, Madrid 28049, Spain

[3] CSIC Cantoblanco, Ctr Nacl Biotecnol, Madrid 28049, Spain

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2009年 / 113卷 / 10期

关键词：

MULTILAMELLAR VESICLES; VISUALIZATION; DELIVERY;

D O I：

10.1021/jp808650e

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Gold nanoparticles have been synthesized inside ethosomes, vesicles composed of phospholipid, ethanol, and water, which could be very efficient not only in delivery probes to the skin but also as diagnostic and therapeutic multimodal agents. High efficiency encapsulation of gold nanoparticles is achieved by a simple strategy: the nanoparticles synthesis occurs simultaneously with the ethosomes formation in the absence of any undesirable reducing agents. A three-dimensional reconstruction of a gold-embedded ethosome generated by cryoelectron tomography reveals that the gold particle is localized inside the lipid bilayer, leaving the ethosome surface and core free for further functionalization. The resulting gold nanoparticles are homogeneous in size and shape and, depending on synthesis temperature, the size ranges from 10 to 20 nm, as revealed by TEM. The ethosome-nanoparticles hybrids' size has been investigated by means of dynamic light scattering and has been found to vary with temperature and gold salt concentration from 700 to 400 nm. Gold nanoparticles-encapsulated ethosomes offer a versatile platform for the enhancement of pharmacological efficacy in transdermal and dermal delivery systems.

引用

页码：3051 / 3057

页数：7