Apoptosis of a human non-small cell lung cancer (NSCLC) cell line, PLA-801, induced by acutiaporberine, a novel bisalkaloid derived from Thalictrum acutifolium (Hand.-Mazz.) Boivin

被引：29

作者：

Chen, Q

Peng, WL

Xu, AL ^{[1
]}

机构：

[1] Zhongshan Univ, Sch Life Sci, Dept Biochem, Guangzhou 510275, Peoples R China

[2] Zhongshan Univ, Sch Life Sci, Ctr Biopharmaceut Res, Guangzhou 510275, Peoples R China

来源：

BIOCHEMICAL PHARMACOLOGY | 2002年 / 63卷 / 08期

基金：

中国国家自然科学基金;

关键词：

acutiaporberine; apoptosis; NSCLC; antitumor drug; Thalictrum acutifolium; TCM;

D O I：

10.1016/S0006-2952(02)00871-7

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Acutiaporberine is a novel other-linked bisalkaloid isolated from the traditional Chinese medicinal herb Thalictrum acutifolium (Hand.-Mazz.) Boivin (TAB). The present study demonstrates for the first time, by means of nuclear staining, DNA agarose gel electrophoresis, and flow cytometry, that acutiaporberine induces apoptosis in PLA-801 cells, a cultured human non-small cell lung cancer (NSCLC) cell line. An immunohistochemical assay and western immunoblot analysis showed down-regulation of the bcl-2 gene and up-regulation of the bax and c-myc genes in the acutiaporberine-treated cells. The observations also indicate that acutiaporberine induces apoptosis of PLA-801 cells in a concentration- and time-dependent manner. These results suggest that acutiaporberine may be a potential, natural apoptosis-inducing agent for NSCLC. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：1389 / 1396

页数：8

共 23 条

[11] TUMOR INHIBITORS .42. THALIDASINE, A NOVEL BISBENZYLISOQUINOLINE ALKALOID TUMOR INHIBITOR FROM THALICTRUM DASYCARPUM [J].