Isolation of Adda from microcystin-LR by microbial degradation

被引：138

作者：

Harada, K ^{[1
]}

Imanishi, S

Kato, H

Mizuno, M

Ito, E

Tsuji, K

机构：

[1] Meijo Univ, Fac Pharm, Nagoya, Aichi 4688503, Japan

[2] Meijo Univ, Grad Sch Environm & Human Sci, Nagoya, Aichi 4688503, Japan

[3] Chiba Univ, Pathogen Fungi & Microbial Toxicoses Res Ctr, Chuo Ku, Chiba 2608673, Japan

[4] Kanagawa Prefectural Inst Publ Hlth, Chigasaki, Kanagawa 2530087, Japan

来源：

TOXICON | 2004年 / 44卷 / 01期

关键词：

microcystin; Adda; microbial degradation; toxicity;

D O I：

10.1016/j.toxicon.2004.04.003

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The intact Adda was isolated from microcystin-LR by a microbial degradation using an isolated Sphingomonas strain, B-9. The reaction of microcystin-LR with cell extract of this strain proceeded smoothly to give the final degradation product by way of two intermediates, linearized microcystin-LR and a tetrapeptide. The purified Adda that was structurally characterized using various spectral data did not show the toxicity to mice or inhibition to protein phosphatase activity in contrast to the native toxin. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：107 / 109

页数：3

共 16 条

[11] Genetic analysis of the peptide synthetase genes for a cyclic heptapeptide microcystin in Microcystis spp. [J].