A highly discriminating quencher-free molecular beacon for probing DNA

被引：166

作者：

Hwang, GT ^{[1
]}

Seo, YJ ^{[1
]}

Kim, BH ^{[1
]}

机构：

[1] Pohang Univ Sci & Technol, Dept Chem, Div Mol & Life Sci, Natl Res Lab, Pohang 790784, South Korea

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 21期

关键词：

D O I：

10.1021/ja049795q

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We inserted a fluorene-labeled deoxyuridine derivative, synthesized using Sonogashira coupling, efficiently into the loop region of a DNA hairpin using phosphoramidite chemistry. This molecular beacon, which features no additional fluorescence quencher, discriminates between perfect and one-base-mismatched base pairing by changes in its fluorescence intensity. The discrimination factor is 14.7 for the recognition of a single (A/C) base mismatch. Copyright © 2004 American Chemical Society.

引用

页码：6528 / 6529

页数：2

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