Protein phosphorylation in response to PDGF stimulation in cultured neurons and astrocytes

被引:10
作者
Zhang, FX
Hutchins, JB
机构
[1] UNIV MISSISSIPPI,MED CTR,DEPT ANAT,JACKSON,MS 39216
[2] UNIV MISSISSIPPI,MED CTR,DEPT NEUROL,JACKSON,MS 39216
来源
DEVELOPMENTAL BRAIN RESEARCH | 1997年 / 99卷 / 02期
关键词
actin-binding protein; cytoskeletal protein; development; growth factor; nervous system; signal transduction; EPIDERMAL GROWTH-FACTOR; CENTRAL-NERVOUS-SYSTEM; MICROVILLAR CORE PROTEIN; SMOOTH-MUSCLE CELLS; ACTIN STRESS FIBERS; FETAL-RAT BRAIN; TYROSINE PHOSPHORYLATION; SIGNAL-TRANSDUCTION; BETA-RECEPTOR; BINDING PROTEIN;
D O I
10.1016/S0165-3806(96)00218-0
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Platelet-derived growth factor (PDGF) is an important growth factor for a variety of cells, including neurons and glial cells. PDGF signal transduction pathways have been studied primarily in mesenchyme-derived cells (such as fibroblasts and smooth muscle cells). However, little is known about these pathways in the central nervous system (CNS). It is believed that phosphorylation is a critical aspect of several steps in the signal transduction pathway. In this study, neurons and type 1 astrocytes in vitro were radiolabeled with P-32-orthophosphate (P-32-P-i). The cells were lysed, and labeled proteins were separated by two-dimensional gel electrophoresis. Autoradiograms of PDGF-stimulated and control samples were compared. We found that in neurons and type 1 astrocytes in vitro, PDGF-BB greatly enhances protein phosphorylation while PDGF-AA has less of an effect on protein phosphorylation. Furthermore, because PDGF signal transduction pathways are likely to affect the cytoskeleton, we studied changes in actin-binding proteins induced by PDGF-BB. We found that PDGF-BB alters the expression, migration pattern and/or avidity of some actin-binding proteins in neurons. In conclusion, protein phosphorylation is up-regulated by PDGF in mouse cortical neurons and type 1 astrocytes in vitro. PDGF's effects on phosphorylation of cytoskeletal proteins might be a important mechanism by which PDGF affects the development and normal functions of central nervous system cells. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:216 / 225
页数:10
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