Taurine protects transformed rat retinal ganglion cells from hypoxia-induced apoptosis by preventing mitochondrial dysfunction

被引:111
作者
Chen, Ka [1 ]
Zhang, Qianyong [1 ]
Wang, Jian [1 ]
Liu, Fengjin [1 ]
Mi, Mantian [1 ]
Xu, Hongxia [1 ]
Chen, Fang [1 ]
Zeng, Kaihong [1 ]
机构
[1] Third Mil Med Univ, Sch Prevent Med, Dept Food Hyg & Nutr, Chongqing 400038, Peoples R China
关键词
Taurine; Hypoxia; Apoptosis; Mitochondria; Retinal ganglion cell line; PERMEABILITY TRANSITION; CALCIUM; NEUROPROTECTION; GLUTAMATE;
D O I
10.1016/j.brainres.2009.04.054
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Hypoxia-induced apoptosis of retinal ganglion cells (RGCs) is the major cause of progressive vision loss in numerous retinal diseases, including glaucoma and diabetic retinopathy. Taurine is a naturally occurring free amino acid that has been shown to have neurotrophic and neuroprotective properties in the retina. We investigated the specific potential for taurine to be protective for immortalized rat retinal ganglion cells (RGC-5) exposed to hypoxia (5% O-2). Pretreatment of RGC-5 cells with 0.1 mM taurine significantly reduced the extent of apoptosis detected by DAPI staining, MTT, and Annexin V-FITC/PI assays, To further study the mechanism underlying the beneficial effect of taurine, interactions between taurine and the process of mitochondria-mediated apoptosis were examined. Taurine treatment of RGC-5 cells suppressed the induction of the mitochondrial permeability transition (mPT) by reducing intracellular calcium levels and inhibiting the opening of mitochondrial permeability transition pores (mPTPs). Moreover, the loss of mitochondrial membrane potential, a decline in cellular ATP levels, a reduction in the amount of cytochrome c translocated to the cytoplasm and caspase-3 activation were observed in taurine-treated cultures. These results demonstrate the potential for taurine to protect RGCs against hypoxic damage in vivo by preventing mitochondrial dysfunction. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:131 / 138
页数:8
相关论文
共 36 条
[21]   Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade [J].
Li, P ;
Nijhawan, D ;
Budihardjo, I ;
Srinivasula, SM ;
Ahmad, M ;
Alnemri, ES ;
Wang, XD .
CELL, 1997, 91 (04) :479-489
[22]   Taurine: Evidence of physiological function in the retina [J].
Militante, JD ;
Lombardini, JB .
NUTRITIONAL NEUROSCIENCE, 2002, 5 (02) :75-90
[23]  
Palmi M, 2006, ADV EXP MED BIOL, V583, P469
[24]  
Palmi M, 2000, ADV EXP MED BIOL, V483, P87
[25]   Taurine and neural cell damage [J].
Saransaari, P ;
Oja, SS .
AMINO ACIDS, 2000, 19 (3-4) :509-526
[26]   Elevation of intracellular Ca2+ concentration induced by hypoxia in retinal ganglion cells [J].
Sasaki, Tsugihisa ;
Kaneko, Akimichi .
JAPANESE JOURNAL OF OPHTHALMOLOGY, 2007, 51 (03) :175-180
[27]   Inducible nitric oxide synthase mediates retinal apoptosis in ischemic proliferative retinopathy [J].
Sennlaub, F ;
Courtois, Y ;
Goureau, O .
JOURNAL OF NEUROSCIENCE, 2002, 22 (10) :3987-3993
[28]   Neuroprotective mechanism of taurine due to up-regulating calpastatin and down-regulating calpain and caspase-3 during focal cerebral ischemia [J].
Ming Sun ;
Chao Xu .
Cellular and Molecular Neurobiology, 2008, 28 (4) :593-611
[29]   Taurine inhibits apoptosis by preventing formation of the Apaf-1/caspase-9 apoptosome [J].
Takatani, T ;
Takahashi, K ;
Uozumi, Y ;
Shikata, E ;
Yamamoto, Y ;
Ito, T ;
Matsuda, T ;
Schaffer, SW ;
Fujio, Y ;
Azuma, J .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2004, 287 (04) :C949-C953
[30]   Taurine prevents the ischemia-induced apoptosis in cultured neonatal rat cardiomyocytes through Akt/caspase-9 pathway [J].
Takatani, T ;
Takahashi, K ;
Uozumi, Y ;
Matsuda, T ;
Ito, T ;
Schaffer, SW ;
Fujio, Y ;
Azuma, J .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 316 (02) :484-489