Characterisation of Fusarium oxysporum isolated from carnation in Australia based on pathogenicity, vegetative compatibility and random amplified polymorphic DNA (RAPD) assay

被引:26
作者
Wright, GFK
Guest, DI
Wimalajeewa, DLS
vanHeeswijck, R
机构
[1] INST PLANT SCI, BURNLEY 3121, AUSTRALIA
[2] UNIV ADELAIDE, DEPT HORT VITICULTURE & OENOL, GLEN OSMOND, SA 5064, AUSTRALIA
关键词
Dianthus caryophyllus; Fusarium wilt;
D O I
10.1007/BF01877139
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Isolates of F. oxysporum collected from symptomless carnation cuttings from Australian carnation growers properties, together with isolates from national collections, were screened for pathogenicity and grouped according to vegetative compatibility and random amplified polymorphic DNA (RAPD) patterns. The collection of 82 Australian isolates sorted into 23 different vegetative compatibility groups (VCGs). Of 69 isolates tested for pathogenicity, 24 were pathogenic to carnations, while the remaining 45 were non-pathogenic. All pathogenic isolates were within two VCGs, one of which was also compatible with an isolate obtained from an international culture collection, and which is known to represent VCG 0021 and race 2. Race status of the two pathogenic VCGs remains unknown. The RAPD assay revealed distinct DNA banding patterns which could distinguish pathogenic from non pathogenic isolates as well as differentiate between isolates from the two pathogenic VCGs.
引用
收藏
页码:451 / 457
页数:7
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