VEGF promotes cardiac stem cells differentiation into vascular endothelial cells via the PI3K/Akt signaling pathway

被引：28

作者：

Xiao, Nuan ^{[1
]}

Qi, Xiao-Yong ^{[1
,2
]}

Tang, Lu-Ning ^{[1
]}

Tan, Li-Li ^{[1
]}

Chen, Ya-Qing ^{[1
]}

Zhao, Hong-Mei ^{[1
]}

机构：

[1] Hebei Med Univ, Dept Internal Med, Shijiazhuang 050017, Peoples R China

[2] Hebei Gen Hosp, Dept Cardiol 1, Shijiazhuang, Peoples R China

来源：

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2014年 / 42卷 / 06期

关键词：

cardiac stem cells; PI3K/Akt signaling pathway; rat; vascular endothelial cells; GROWTH-FACTOR; THERAPEUTIC ANGIOGENESIS; CARDIOVASCULAR-DISEASE; ORGAN GROWTH; AKT; ACTIVATION; MECHANISMS; DROSOPHILA;

D O I：

10.3109/21691401.2013.837473

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recent research suggested that cardiac stem cells (CSCs) may have the clinical application for cardiac repair. However, their characteristics and the regulatory mechanisms of their growth and differentiation have not been fully investigated. Vascular endothelial growth factor (VEGF, VEGF-A) is a major regulator of physiological and pathological angiogenesis. But the homing role of VEGF for CSCs is unclear. In this report, CSCs were isolated, purified, and expanded in vitro from rat heart. VEGF, SU5416 (VEGF receptor blocker), and Wortmannin (PI3K/Akt signaling pathway inhibitor) were used for differentiation into vascular endothelial cells (VECs). Real-time qPCR was selected to confirm the role of PI3K/Akt signaling pathway in VECs differentiation from rat CSCs. The result of real-time qPCR demonstrated that PI3K/Akt signaling pathway plays an important role in rat CSCs differentiated into VECs. So, our research provides a theoretical basis and experimental evidence for therapeutic application of rat CSCs to treat cardiac repair.

引用

页码：400 / 405

页数：6

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