Sensitive biomolecule detection in lateral flow assay with a portable temperature-humidity control device

被引：76

作者：

Choi, Jane Ru ^{[1
,2
,3
]}

Hu, Jie ^{[2
,3
]}

Feng, Shangsheng ^{[2
,4
,5
]}

Abas, Wan Abu Bakar Wan ^{[1
]}

Pingguan-Murphy, Belinda ^{[1
]}

Xu, Feng ^{[2
,3
]}

机构：

[1] Univ Malaya, Fac Engn, Dept Biomed Engn, Kuala Lumpur 50603, Malaysia

[2] Xi An Jiao Tong Univ, Sch Life Sci & Technol, Key Lab Biomed Informat Engn, Minist Educ, Xian 710049, Peoples R China

[3] Xi An Jiao Tong Univ, BEBC, Xian 710049, Peoples R China

[4] Xi An Jiao Tong Univ, Sch Aerosp, MOE Key Lab Multifunct Mat & Struct LMMS, Xian 710049, Peoples R China

[5] Xi An Jiao Tong Univ, Sch Aerosp, State Key Lab Mech Struct Strength & Vibrat, Xian 710049, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2016年 / 79卷

基金：

对外科技合作项目（国际科技项目）;

关键词：

LFA; POC; Nucleic acid hybridization; Antigen-antibody interaction; Temperature; RH; Portable temperature-humidity control device; ENHANCED SENSITIVITY; POINT; PCR; PLATFORM; LABEL; STRIP;

D O I：

10.1016/j.bios.2015.12.005

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Lateral flow assays (LFAs) have currently attracted broad interest for point-of-care (POC) diagnostics, but their application has been restricted by poor quantification and limited sensitivity. While the former has been currently solved to some extent by the development of handheld or smartphone-based readers, the latter has not been addressed fully, particularly the potential influences of environmental conditions (e.g., temperature and relative humidity (RH)), which have not yet received serious attention. The present study reports the use of a portable temperature-humidity control device to provide an optimum environmental requirement for sensitivity improvement in LFAs, followed by quantification by using a smartphone. We found that a RH beyond 60% with temperatures of 55-60 degrees C and 37-40 degrees C produced optimum nucleic acid hybridization and antigen-antibody interaction in LFAs, respectively representing a 10-fold and 3-fold signal enhancement over ambient conditions (25 degrees C, 60% RH). We envision that in the future the portable device could be coupled with a fully integrated paper-based sample-to-answer biosensor for sensitive detection of various target analytes in POC settings. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：98 / 107

页数：10

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