Dimerization of Arabidopsis 14-3-3 proteins:: structural requirements within the N-terminal domain and effect of calcium

被引：23

作者：

Abarca, D ^{[1
]}

Madueño, F ^{[1
]}

Martínez-Zapater, JM ^{[1
]}

Salinas, J ^{[1
]}

机构：

[1] INIA, Dept Mejora Genet & Biotecnol, Madrid 28040, Spain

来源：

FEBS LETTERS | 1999年 / 462卷 / 03期

关键词：

14-3-3; protein; protein dimerization; calcium; Arabidopsis;

D O I：

10.1016/S0014-5793(99)01560-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structural requirements for dimerization of RCI14A and RCI14B, two 14-3-3 isoforms from Arabidopsis thaliana, have been analyzed by testing truncated forms of RCI14A for dimerization with full-length RCI14A and RCI14B, The results show that only the fourth helix of the truncated partner is essential for dimerization, which represents a difference from what is known for animal isoforms, On the other hand, the effect of calcium has been tested in RCI14A homodimerization. Millimolar concentrations of calcium exert a negative, dose-dependent effect that involves the C-terminal domain of RCI14A and might modulate interactions with other cellular components or among Arabidopsis 14-3-3 isoforms, (C) 1999 Federation of European Biochemical Societies.

引用

页码：377 / 382

页数：6

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