Investigation of flavonoid profile of Scutellaria bacalensis Georgi by high performance liquid chromatography with diode array detection and electrospray ion trap mass spectrometry

被引：130

作者：

Liu, Guozhu ^{[1
]}

Ma, Jinyu ^{[1
]}

Chen, Yingzhuang ^{[1
]}

Tian, Qingqing ^{[1
]}

Shen, Yao ^{[1
]}

Wang, Xiaosong ^{[1
]}

Chen, Bo ^{[1
]}

Yao, Shouzhuo ^{[1
]}

机构：

[1] Hunan Normal Univ, Key Lab Chem Biol & Tradit Chinese Med Res, Minist Educ, Changsha 410081, Hunan, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 23期

基金：

中国国家自然科学基金;

关键词：

Scutellaria baicalensis Georgi; Flavonoids; Profile; HPLC-UV/MS; BAICALENSIS;

D O I：

10.1016/j.chroma.2009.04.021

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Scutellaria baicalensis Georgi is a well-known medicinal plant widely used in China and other East Asian countries. High performance liquid chromatography combined with diode array detection and electrospray ion trap mass spectrometry Was used to determine the flavonoid profile of S. baicalensis. Under the optimized experiment conditions, 32 flavonoids were clearly detected. Eighteen main ones were doubtless identified by comparing their retention time, UV and NIS (MSn) data with isolated or commercial standards. The UV characteristics of these 18 known standards were studied in derail. The rules summarized provided valuable indications for the Subsequent on-line identification processes. By interpreting both the MS and the UV data in detail, other 13 minor flavonoids in S. baicalensis were on-line identified successfully. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：4809 / 4814

页数：6

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