Virosome-mediated delivery of protein antigens to dendritic cells

被引：106

作者：

Bungener, L

Serre, K

Bijl, L

Leserman, L

Wilschut, J

Daemen, T ^{[1
]}

Machy, P

机构：

[1] Univ Groningen, Dept Med Microbiol, Mol Virol Sect, Groningen, Netherlands

[2] Ctr Immunol Marseille Luminy, F-13288 Marseille 9, France

来源：

VACCINE | 2002年 / 20卷 / 17-18期

关键词：

virosomes; dendritic; cells; antigen presentation;

D O I：

10.1016/S0264-410X(02)00103-2

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Virosomes are reconstituted viral membranes in which protein can be encapsulated. Fusion-active virosomes, fusion-inactive virosomes and liposomes were used to study the conditions needed for delivery of encapsulated protein antigen ovalbumin (OVA) to dendritic cells (DCs) for MHC class I and 11 presentation. Fusion-active virosomes, but not fusion-inactive virosomes, were able to deliver OVA to DCs for MHC class I presentation at picomolar OVA concentrations. Fusion activity of virosomes was not required for MHC class H presentation of antigen. Therefore, virosomes are an efficient system for delivery of protein antigens for stimulation of both helper and CTL responses. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：2287 / 2295

页数：9

共 38 条

[1] Induction of cytotoxic T lymphocyte activity by fusion-active peptide-containing virosomes [J].