Amplification of MGC2177, PLAG1, PSMC6P, and LYN in a malignant mixed tumor of salivary gland detected by cDNA microarray with tyramide signal amplification

被引：9

作者：

Tsang, YTM ^{[1
]}

Chang, YM ^{[1
]}

Lu, XY ^{[1
]}

Rao, PH ^{[1
]}

Lau, CC ^{[1
]}

Wong, KK ^{[1
]}

机构：

[1] Baylor Coll Med, Texas Childrens Canc Ctr, Canc Genom Grp, Dept Pediat, Houston, TX 77030 USA

来源：

CANCER GENETICS AND CYTOGENETICS | 2004年 / 152卷 / 02期

关键词：

D O I：

10.1016/j.cancergencyto.2003.12.001

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Gene amplifications have been observed in many different tumor cells, and many of these changes are related to tumor pathogenesis. Comparative genomic hybridization (CGH) using metaphase chromosomes can detect changes in chromosome copy number with a resolution of 10-20 Mb. Current advances in CGH analysis in a microarray format allow us to refine such changes down to the gene level. We applied microarray technology to detect novel gene amplification in a malignant mixed tumor of salivary gland. Besides detecting previously known gene amplifications (MDM2 and MYC), we identified four other highly amplified genes located at 8q11.2similar toq13: MGC2177, PLAG1, PSMC6P, and LYN. The amplification was further validated with real-time quantitative polymerase chain reaction. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：124 / 128

页数：5

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