Large area protein nanopatterning for biological applications

被引：105

作者：

Agheli, H.

Malmstrom, J.

Larsson, E. M.

Textor, M.

Sutherland, D. S. ^{[1
]}

机构：

[1] Chalmers Univ Technol, Dept Appl Phys, S-41296 Gothenburg, Sweden

[2] Univ Aarhus, iNANO Ctr, DK-8000 Aarhus, Denmark

[3] ETH, Lab Surface Sci & Technol, Dept Mat, CH-8093 Zurich, Switzerland

来源：

NANO LETTERS | 2006年 / 6卷 / 06期

关键词：

D O I：

10.1021/nl060403i

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Large area nanopatterns of functional proteins are demonstrated. A new approach to analyze atomic force microscopy height histograms is used to quantify protein and antibody binding to nanoscale patches. Arrays of nanopatches, each containing less than 40 laminin molecules, are shown to be highly functional binding close to 1 monoclonal anti-laminin IgG (site by IKVAV sequence) or 3-4 polyclonal anti-laminin IgG's per surface bound laminin. Complementary quartz crystal microbalance measurements indicate higher functionality at nanopatches than on homogeneous surfaces.

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收藏

页码：1165 / 1171

页数：7

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