Identification of cell lines permissive for human coronavirus NL63

被引：34

作者：

Schildgen, Oliver

Jebbink, Maarten F.

de Vries, Michel

Pyrc, Krzysztov

Dijkman, Ronald

Simon, Arne

Mueller, Andreas

Kupfer, Bernd

van der Hoek, Lia

机构：

[1] Univ Bonn, Inst Med Microbiol Immunol & Parasitol, Dept Virol, D-53105 Bonn, Germany

[2] Univ Amsterdam, Acad Med Ctr, Lab Expt Virol, Dept Med Microbiol,Ctr Infect & Immun, NL-1012 WX Amsterdam, Netherlands

[3] Univ Bonn, Childrens Hosp, D-5300 Bonn, Germany

来源：

JOURNAL OF VIROLOGICAL METHODS | 2006年 / 138卷 / 1-2期

关键词：

human coronavirus; NL63; cell culture; permissive cell lines;

D O I：

10.1016/j.jviromet.2006.07.023

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Six cell lines routinely used in laboratories were tested for permissiveness to the infection with the newly identified human coronavirus NL63. Two monkey epithelial cell lines, LLC-MK2 and Vero-134, showed a cytopathic effect (CPE) and clear viral replication, whereas no CPE or replication was observed in human lung fibroblasts MRC-5s. In Rhabdomyosarcoma cells, Madin-Darby-Canine-kidney cells and in an undefined monkey kidney cell line some replication was observed but massive exponential rise in virus yield lacked The results will lead to an improved routine diagnostic algorithm for the detection of the human coronavirus NL63. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：207 / 210

页数：4

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