Development of a Robust and Repeatable UPLC-MS Method for the Long-Term Metabolomic Study of Human Serum

被引：514

作者：

Zelena, Eva ^{[4
]}

Dunn, Warwick B. ^{[3
]}

Broadhurst, David ^{[4
]}

Francis-McIntyre, Sue ^{[4
]}

Carroll, Kathleen M. ^{[3
]}

Begley, Paul ^{[4
]}

O'Hagan, Steve ^{[4
]}

Knowles, Joshua D. ^{[1
]}

Halsall, Antony ^{[4
]}

Wilson, Ian D. ^{[2
]}

Kellt, Douglas B. ^{[3
,4
]}

机构：

[1] Univ Manchester, Sch Comp Sci, Manchester Interdisciplinary Bioctr, Manchester M1 7DN, Lancs, England

[2] AstraZeneca, Dept Clin Pharmacol Drug Metab & Pharmacokinet, Macclesfield SK10 4TG, Cheshire, England

[3] Univ Manchester, Sch Chem, Manchester Interdisciplinary Bioctr, Manchester Ctr Integrat Syst Biol, Manchester M1 7DN, Lancs, England

[4] Univ Manchester, Bioanalyt Sci Grp, Manchester M1 7DN, Lancs, England

来源：

ANALYTICAL CHEMISTRY | 2009年 / 81卷 / 04期

基金：

英国生物技术与生命科学研究理事会;

关键词：

PERFORMANCE LIQUID-CHROMATOGRAPHY; FLIGHT MASS-SPECTROMETRY; SYSTEMS BIOLOGY; METABONOMIC ANALYSIS; PLANT METABOLOMICS; HUMAN URINE; HPLC-MS; BIOMARKERS; STRATEGY; IDENTIFICATION;

D O I：

10.1021/ac8019366

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for performing untargeted metabolomic analysis of human serum has been developed based on protein precipitation followed by Ultra Performance liquid Chromatography and Time-of-Flight mass spectrometry (UPLC-TOF-MS). This method was specifically designed to fulfill the requirements of a long-term metabolomic study, spanning more than 3 years, and it was subsequently thoroughly evaluated for robustness and repeatability. We describe here the observed drift in instrumental performance over time and its improvement with adjustment of the length of analytical block. The optimal setup for our purpose was further validated against a set of serum samples from 30 healthy individuals. We also assessed the reproducibility of chromatographic columns with the same chemistry of stationary phase from the same manufacturer but from different production batches. The results have allowed the authors to prepare SON for "fit for purpose" long-term UPLC-MS metabolomic studies, such as are being employed in the HUSERMET project. This method allows the acquisition of data and subsequent comparison of data collected across many months or years.

引用

页码：1357 / 1364

页数：8

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