Phosphomonoester concentrations differ between chronic lymphocytic leukemia cells and normal human lymphocytes

被引：27

作者：

Franks, SE ^{[1
]}

Smith, MR ^{[1
]}

Arias-Mendoza, F ^{[1
]}

Shaller, C ^{[1
]}

Padavic-Shaller, K ^{[1
]}

Kappler, F ^{[1
]}

Zhang, Y ^{[1
]}

Negendank, WG ^{[1
]}

Brown, TR ^{[1
]}

机构：

[1] Fox Chase Canc Ctr, Philadelphia, PA 19111 USA

来源：

LEUKEMIA RESEARCH | 2002年 / 26卷 / 10期

关键词：

phosphomonoesters; B-cell malignancies; MR spectroscopy; chronic lymphocytic leukemia; non-hodgkin's lymphoma;

D O I：

10.1016/S0145-2126(02)00035-8

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Levels of phospholipid-related metabolites of chronic lyniphocytic leukemia lymphocytes (CLL) and normal human lymphocytes were quantified using phosphorus magnetic resonance spectroscopy. The CLL cells versus normal lymphocytes showed significant increases of phosphoethanolamine(Etn-P) (8.11 +/- 2.10 mean +/- S.E., mumol/g wet weight, n = 12 versus 3.63 +/- 1.10, n = 3, P < 0.002), phosphocholine (2.10 +/- 0.37, n = 12 versus 0.36 +/- 0.09, n = 3, P less than or equal to 0.01), glycerophosphoethanolarnine (0.26 +/- 0.03, n = 10 versus 0.11 +/- 0.05, n = 3 P less than or equal to 0.004). and glycerophosphocholine (0.33 +/- 0.03, n = 10 versus 0. 17 +/- 0.05, n = 3, P less than or equal to 0.003). Further, the phospholipid precursor ethanolamine (Eth) was studied in blood and was found significantly lowered in CLL patients (4.6 +/- 1 6 muM. n = 25) compared to normal Volunteers (7.7 +/- 2.5, n = 12, P less than or equal to 0.001). Increased intermediate,, with depletion of precursors suggest the presence of sustained phospholipid metabolism activation in CLL. (C) 2002 Published by Elsevier Science Ltd.

引用

页码：919 / 926

页数：8

共 58 条

[41] PHOSPHOLIPID BASE-EXCHANGE IN HUMAN-LEUKOCYTE MEMBRANES - QUANTITATION AND CORRELATION WITH OTHER PHOSPHOLIPID BIOSYNTHETIC PATHWAYS [J].