1,25 dihydroxyvitamin D-3 stimulates phospholipase C-gamma in rat colonocytes: Role of c-Src in PLC-gamma activation

被引:62
作者
Khare, S [1 ]
Bolt, MJG [1 ]
Wali, RK [1 ]
Skarosi, SF [1 ]
Boy, HK [1 ]
Niedziela, S [1 ]
ScaglioneSewell, B [1 ]
Aquino, B [1 ]
Abraham, C [1 ]
Sitrin, MD [1 ]
Brasitus, TA [1 ]
Bissonnette, M [1 ]
机构
[1] UNIV CHICAGO, DEPT MED, CHICAGO, IL 60637 USA
关键词
1,25-dihydroxycholecalciferol; nonreceptor tyrosine kinase; basolateral membrane; vitamin D deficiency;
D O I
10.1172/JCI119350
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Our laboratory has previously demonstrated that 1,25-dihydroxyvitamin D-3 (1,25[OH](2)D-3) rapidly stimulated polyphosphoinositide (PI) hydrolysis, raised intracellular Ca2+, and activated two Ca2+-dependent protein kinase C (PKC) isoforms, PKC-alpha and -beta(II) in the rat large intestine. We also showed that the direct addition of 1,25(OH)(2)D-3 to isolated colonic membranes failed to stimulate PI hydrolysis, but required secosteroid treatment of intact colonocytes, suggesting the involvement of a soluble factor. Furthermore, this PI hydrolysis was restricted to the basal lateral plasma membrane of these cells. In the present studies, therefore, we examined whether polyphosphoinositide-phospholipase C-gamma (PI-PLC-gamma), a predominantly cytosolic isoform of PI-PLC, was involved in the hydrolysis of colonic membrane PI by 1,25(OH)(2)D-3. This isoform has been shown to be activated and membrane-associated by tyrosine phosphorylation. We found that 1,25(OH)(2)D-3 caused a significant increase in the biochemical activity, particulate association, and the tyrosine phosphorylation of PLC-gamma, specifically in the basal lateral membranes. This secosteroid also induced a twofold increase in the activity of Src, a proximate activator of PLC-gamma in other cells, with peaks at 1 and 9 min in association with Src tyrosine dephosphorylation. 1,25(OH)(2)D-3 also increased the physical association of activated c-Src with PLC-gamma. In addition, Src isolated from colonocytes treated with 1,25(OH)(2)D-3, demonstrated an increased ability to phosphorylate exogenous PLC-gamma in vitro. Inhibition of 1,25(OH)(2)D-3-induced Src activation by PP1, a specific Src family protein tyrosine kinase inhibitor, blocked the ability of this secosteroid to stimulate the translocation and tyrosine phosphorylation of PLC-gamma in the basolateral membrane (BLM). Src activation was last in D deficiency, and was reversibly restored with the in vivo repletion of 1,25(OH)(2)D-3 These studies demonstrate for the first time that 1,25(OH)(2)D-3 stimulates PLC-gamma as well as c-Src in rat colonocytes, and indicate that PLC-gamma is a direct substrate of secosteroid-activated c-Src in these cells.
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收藏
页码:1831 / 1841
页数:11
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