1,25 dihydroxyvitamin D-3 stimulates phospholipase C-gamma in rat colonocytes: Role of c-Src in PLC-gamma activation

被引：62

作者：

Khare, S ^{[1
]}

Bolt, MJG ^{[1
]}

Wali, RK ^{[1
]}

Skarosi, SF ^{[1
]}

Boy, HK ^{[1
]}

Niedziela, S ^{[1
]}

ScaglioneSewell, B ^{[1
]}

Aquino, B ^{[1
]}

Abraham, C ^{[1
]}

Sitrin, MD ^{[1
]}

Brasitus, TA ^{[1
]}

Bissonnette, M ^{[1
]}

机构：

[1] UNIV CHICAGO, DEPT MED, CHICAGO, IL 60637 USA

来源：

JOURNAL OF CLINICAL INVESTIGATION | 1997年 / 99卷 / 08期

关键词：

1,25-dihydroxycholecalciferol; nonreceptor tyrosine kinase; basolateral membrane; vitamin D deficiency;

D O I：

10.1172/JCI119350

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Our laboratory has previously demonstrated that 1,25-dihydroxyvitamin D-3 (1,25[OH](2)D-3) rapidly stimulated polyphosphoinositide (PI) hydrolysis, raised intracellular Ca2+, and activated two Ca2+-dependent protein kinase C (PKC) isoforms, PKC-alpha and -beta(II) in the rat large intestine. We also showed that the direct addition of 1,25(OH)(2)D-3 to isolated colonic membranes failed to stimulate PI hydrolysis, but required secosteroid treatment of intact colonocytes, suggesting the involvement of a soluble factor. Furthermore, this PI hydrolysis was restricted to the basal lateral plasma membrane of these cells. In the present studies, therefore, we examined whether polyphosphoinositide-phospholipase C-gamma (PI-PLC-gamma), a predominantly cytosolic isoform of PI-PLC, was involved in the hydrolysis of colonic membrane PI by 1,25(OH)(2)D-3. This isoform has been shown to be activated and membrane-associated by tyrosine phosphorylation. We found that 1,25(OH)(2)D-3 caused a significant increase in the biochemical activity, particulate association, and the tyrosine phosphorylation of PLC-gamma, specifically in the basal lateral membranes. This secosteroid also induced a twofold increase in the activity of Src, a proximate activator of PLC-gamma in other cells, with peaks at 1 and 9 min in association with Src tyrosine dephosphorylation. 1,25(OH)(2)D-3 also increased the physical association of activated c-Src with PLC-gamma. In addition, Src isolated from colonocytes treated with 1,25(OH)(2)D-3, demonstrated an increased ability to phosphorylate exogenous PLC-gamma in vitro. Inhibition of 1,25(OH)(2)D-3-induced Src activation by PP1, a specific Src family protein tyrosine kinase inhibitor, blocked the ability of this secosteroid to stimulate the translocation and tyrosine phosphorylation of PLC-gamma in the basolateral membrane (BLM). Src activation was last in D deficiency, and was reversibly restored with the in vivo repletion of 1,25(OH)(2)D-3 These studies demonstrate for the first time that 1,25(OH)(2)D-3 stimulates PLC-gamma as well as c-Src in rat colonocytes, and indicate that PLC-gamma is a direct substrate of secosteroid-activated c-Src in these cells.

引用

页码：1831 / 1841

页数：11

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