Biodegradation of indole at high concentration by persolvent fermentation with Pseudomonas sp. ST-200

被引:46
作者
Doukyu, N
Aono, R
机构
[1] Department of Bioengineering, Fac. of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226
关键词
biodegradation of indole; indole; Pseudomonas sp; persolvent fermentation; organic solvent;
D O I
10.1007/s007920050021
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Pseudomonas sp. strain ST-200 grew on indole as a sole carbon source. The minimal inhibitory concentration of indole was 0.3 mg/ml for ST-200. However, ST-200 grew in a persolvent fermentation system containing a large amount of indole (a medium containing 20% by vol. diphenylmethane and 4 mg/ml indole), because most of the indole was partitioned in the organic solvent layer. When the organism was grown in the medium containing indole at 1 mg/ml in the presence of diphenyl-methane, more than 98% of the indole was consumed after 48h. Isatic acid (0.4 mg/ml) and isatin (0.03 mg/ml) were produced as the metabolites in the aqueous medium layer.
引用
收藏
页码:100 / 105
页数:6
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