Human and Mouse Skeletal Muscle Stem Cells: Convergent and Divergent Mechanisms of Myogenesis

被引：64

作者：

Bareja, Akshay ^{[1
,2
]}

Holt, Jason A. ^{[2
]}

Luo, Guizhen ^{[2
]}

Chang, Calvin ^{[3
]}

Lin, Junyu ^{[3
]}

Hinken, Aaron C. ^{[3
]}

Freudenberg, Johannes M. ^{[4
]}

Kraus, William E. ^{[1
]}

Evans, William J. ^{[2
]}

Billin, Andrew N. ^{[2
]}

机构：

[1] Duke Univ, Dept Med, Durham, NC USA

[2] GlaxoSmithKline, Metab Pathways & Cardiovasc Therapeut Area, Muscle Metab Discovery Performance Unit, Res Triangle Pk, NC 27709 USA

[3] Five Prime Therapeut Inc, San Francisco, CA USA

[4] GlaxoSmithKline, Computat Biol, Quantitat Sci, Res Triangle Pk, NC USA

来源：

PLOS ONE | 2014年 / 9卷 / 02期

关键词：

SATELLITE CELLS; PROGENITOR CELLS; SELF-RENEWAL; EXPRESSION; MYF5; DIFFERENTIATION; RECRUITMENT; MARKER;

D O I：

10.1371/journal.pone.0090398

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Satellite cells are the chief contributor to skeletal muscle growth and regeneration. The study of mouse satellite cells has accelerated in recent years due to technical advancements in the isolation of these cells. The study of human satellite cells has lagged and thus little is known about how the biology of mouse and human satellite cells compare. We developed a flow cytometry-based method to prospectively isolate human skeletal muscle progenitors from the satellite cell pool using positive and negative selection markers. Results show that this pool is enriched in PAX7 expressing cells that possess robust myogenic potential including the ability to give rise to de novo muscle in vivo. We compared mouse and human satellite cells in culture and identify differences in the elaboration of the myogenic genetic program and in the sensitivity of the cells to cytokine stimulation. These results indicate that not all mechanisms regulating mouse satellite cell activation are conserved in human satellite cells and that such differences may impact the clinical translation of therapeutics validated in mouse models. Thus, the findings of this study are relevant to developing therapies to combat muscle disease.

引用

页数：12