Zebrafish Trap230/Med12 is required as a coactivator for Sox.9-dependent neural crest, cartilage and ear development

被引：74

作者：

Rau, Marlene J. ^{[1
]}

Fischer, Sabine ^{[1
]}

Neumann, Carl J. ^{[1
]}

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

来源：

DEVELOPMENTAL BIOLOGY | 2006年 / 296卷 / 01期

关键词：

zebrafish; development; neural crest; limb; Mediator; TRAP230; MED12; Sox9; chondrogenesis; pectoral fins; trapped;

D O I：

10.1016/j.ydbio.2006.04.437

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The vertebrate Sox9 transcription factor directs the development of neural crest, otic placodes, cartilage and bone. In zebrafish, there are two Sox9 orthologs, Sox9a and Sox9b, which together perform the functions of the single-copy tetrapod Sox9. In a large-scale genetic screen, we have identified a novel zebrafish mutant that strongly resembles the Sox9a/Sox9b double mutant phenotype. We show that this mutation disrupts the zebrafish Trap230/Med 12 ortholog, a member of the Mediator complex. Mediator is a coactivator complex transducing the interaction of DNA-binding transcription factors with RNA polymerase II, and our results reveal a critical function of the Trap230 subunit as a coactivator for Sox9. (c) 2006 Elsevier Inc. All rights reserved.

引用

收藏

页码：83 / 93

页数：11

相关论文

共 48 条

[1] T-box gene tbx5 is essential for formation of the pectoral limb bud [J].

Ahn, DG ;

Kourakis, MJ ;

Rohde, LA ;

Silver, LM ;

Ho, RK .

NATURE, 2002, 417 (6890) :754-758

[2] The transcrintion factor Sox9 has essential roles in successive steps of the chondrocyte differentiation pathway and is required for expression of Sox5 and Sox6 [J].

Akiyama, H ;

Chaboissier, MC ;

Martin, JF ;

Schedl, A ;

de Crombrugghe, B .

GENES & DEVELOPMENT, 2002, 16 (21) :2813-2828

[3] Interactions between Sox9 and β-catenin control chondrocyte differentiation [J].

Akiyama, H ;

Lyons, JP ;

Mori-Akiyama, Y ;

Yang, XH ;

Zhang, R ;

Zhang, ZP ;

Deng, JM ;

Taketo, MM ;

Nakamura, T ;

Behringer, RR ;

McCrea, PD ;

de Crombrugghe, B .

GENES & DEVELOPMENT, 2004, 18 (09) :1072-1087

[4] SOX9 directly regulates the type-II collagen gene [J].

Bell, DM ;

Leung, KKH ;

Wheatley, SC ;

Ng, LJ ;

Zhou, S ;

Ling, KW ;

Sham, MH ;

Koopman, P ;

Tam, PPL ;

Cheah, KSE .

NATURE GENETICS, 1997, 16 (02) :174-178

[5] Sox9 is required for cartilage formation [J].

Bi, WM ;

Deng, JM ;

Zhang, ZP ;

Behringer, RR ;

de Crombrugghe, B .

NATURE GENETICS, 1999, 22 (01) :85-89

[6] The yeast Mediator complex and its regulation [J].

Björklund, S ;

Gustafsson, CM .

TRENDS IN BIOCHEMICAL SCIENCES, 2005, 30 (05) :240-244

[7] Drosophila homologs of transcriptional mediator complex subunits are required for adult cell and segment identity specification [J].

Boube, M ;

Faucher, C ;

Joulia, L ;

Cribbs, DL ;

Bourbon, HM .

GENES & DEVELOPMENT, 2000, 14 (22) :2906-2917

[8] Neural crest development is regulated by the transcription factor Sox9 [J].

Cheung, M ;

Briscoe, J .

DEVELOPMENT, 2003, 130 (23) :5681-5693

[9] The transcriptional control of trunk neural crest induction, survival, and delamination [J].

Cheung, M ;

Chaboissier, MC ;

Mynett, A ;

Hirst, E ;

Schedl, A ;

Briscoe, J .

DEVELOPMENTAL CELL, 2005, 8 (02) :179-192

[10] The zebrafish fgf24 mutant identifies an additional level of Fgf signaling involved in vertebrate forelimb initiation [J].

Fischer, S ;

Draper, BW ;

Neumann, CJ .

DEVELOPMENT, 2003, 130 (15) :3515-3524

← 1 2 3 4 5 →