Quantification of Saccharomyces cerevisiae viability using BacLight

被引：36

作者：

Zhang, T ^{[1
]}

Fang, HHP ^{[1
]}

机构：

[1] Univ Hong Kong, Dept Civil Engn, Environm Biotechnol Lab, Hong Kong, Hong Kong, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 12期

关键词：

BacLight; cell; Saccharomyces cerevisiae; viability; yeast;

D O I：

10.1023/B:BILE.0000030045.16713.19

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Yeast viability can be accurately quantified using BacLight, a kit which so far has been used only for bacterial analysis. Upon staining, viable cells can be differentiated from non-viable ones by either confocal laser scanning microscopy (CLSM), epifluorescence microscopy, or flow cytometry. Using Saccharomyces cerevisiae as a model, viabilities quantified by CLSM deviated an average of 1.7% from the actual data, and those determined by flow-cytometry by 1.4%.

引用

页码：989 / 992

页数：4

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