A flow-cytometric method for determination of yeast viability and cell number in a brewery

被引：18

作者：

Boyd, AR ^{[1
]}

Gunasekera, TS

Attfield, PV

Simic, K

Vincent, SF

Veal, DA

机构：

[1] Macquarie Univ, Dept Biol Sci, Ctr Fluorimetr Applicat Biotechnol, N Ryde, NSW 2109, Australia

[2] Carlton & United Breweries, Kent Brewery, Sydney, NSW 2000, Australia

来源：

FEMS YEAST RESEARCH | 2003年 / 3卷 / 01期

基金：

澳大利亚研究理事会;

关键词：

flow cytometry; oxonol; viability; pitching rate; Saccharomyces carlsbergensis;

D O I：

10.1016/S1567-1356(02)00125-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A flow-cytometric assay, using the fluorescent dye, oxonol, for the simultaneous determination of yeast cell viability and cell number is described. The assay was optimised, and trialed at a brewery for 6 months. The flow-cytometry assay offered a substantially reduced error in viability determination, compared to methylene blue which is the industry standard for measuring viability. Further, by calculating yeast cell number at the same time, this assay provides a reliable method for determining pitching rate, allowing increased quality control of subsequent fermentations. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：11 / 16

页数：6

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