Integration host factor interactions with Neisseria gene sequences:: correlation between predicted binding sites and in vitro binding of Neisseria-derived IHF protein

被引：6

作者：

Hill, SA ^{[1
]}

Samuels, DS

Nielsen, C

Knight, SW

Pagotto, F

Dillon, JAR

机构：

[1] No Illinois Univ, Dept Biol Sci, De Kalb, IL 60115 USA

[2] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA

[3] Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa, ON K1H 8M5, Canada

[4] Univ Ottawa, Ctr Res Biopharmaceut, Ottawa, ON K1H 8M5, Canada

来源：

MOLECULAR AND CELLULAR PROBES | 2002年 / 16卷 / 02期

关键词：

integration host factor; Neisseria; gel retardation assays;

D O I：

10.1006/mcpr.2001.0403

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Putative integration host factor (IHF) binding sites are frequently being identified in Neisseria gene sequences on the basis of similarity to a degenerate Escherichia coli-derived consensus binding sequence. In this report, three different Neisseria genetic systems that contain predicted IHF binding sites were assessed for IHF binding through gel retardation analysis. The results show a positive correlation between the identification of a predicted Neisseria IHF binding site and in vitro binding of Neisseria-derived IHF protein. (C) 2002 Elsevier Science Ltd.

引用

页码：153 / 158

页数：6

共 26 条

[11] Capsule phase variation in Neisseria meningitidis serogroup B by slipped-strand mispairing in the polysialyltransferase gene (siaD): Correlation with bacterial invasion and the outbreak of meningococcal disease [J].