Lipase immobilized on poly(VP-co-HEMA) hydrogel for esterification reaction

被引：11

作者：

Basri, M ^{[1
]}

Samsudin, S ^{[1
]}

Bin Ahmad, M ^{[1
]}

Razak, CNA ^{[1
]}

Salleh, AB ^{[1
]}

机构：

[1] Univ Putra Malaysia, Fak Sains & Pengajian Alam Sekitar, Ctr Res Enzyme & Microbial Technol Jabatan Kimi, Serdang 43400, Malaysia

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1999年 / 81卷 / 03期

关键词：

lipase; immobilization; hydrogel; esterification; stability;

D O I：

10.1385/ABAB:81:3:205

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipase from Candida rugosa was immobilized by entrapment on poly(N-vinyl-2-pyrrolidone-co-2-hydroxyethylmethacrylate) (poly[VP-co-HEMA]) hydrogel, and divinylbenzene was the crosslinking agent. The immobilized enzymes were used in the esterification reaction of oleic acid and butanol in hexane. The activities of the immobilized enzymes and the leaching ability of the enzyme from the support with respect to the different compositions of the hydrogels were investigated. The thermal, solvent, and storage stability of the immobilized lipases was also determined. increasing the percentage of composition of VP from 0 to 90, which corresponds to the increase in the hydrophilicity of the hydrogels, increased the activity of the immobilized enzyme. Lipase immobilized on VP(%):HEMA(%) 90:10 exhibited the highest activity. Lipase immobilized on VP(%):HEMA(%) 50:50 showed the highest thermal, solvent, storage, and operational stability compared to lipase immobilized on other compositions of hydrogels as well as the native lipase.

引用

页码：205 / 217

页数：13

共 10 条

[1] ENZYMATIC-SYNTHESIS OF FATTY ESTERS BY HYDROPHOBIC LIPASE DERIVATIVES IMMOBILIZED ON ORGANIC POLYMER BEADS [J].